prfB

168

peptide chain release factor 2, recognize stop codon and hydrolyze the completed protein, RF2 recognizes UAA and UGA

locus: BSU_35290

Molecular weight: 41.91 kDa

pI: 4.85

Protein length: 366 aa

Gene length: 1102 bp

function: translation

product: peptide chain release factor 2

essential: yes

synonyms: prfB

List of homologs in different organisms, belongs to COG1186 (Galperin et al., 2021)

Gene

Coordinates: 3,627,139  3,628,240

Phenotypes of a mutant

• essential [Pubmed|12682299]

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The protein

Catalyzed reaction/ biological activity

• binds to the ribosome through specific recognition of messenger RNA stop codons and triggers hydrolysis of the bond between the nascent polypeptide and the transfer RNA at the peptidyl-tRNA site, thereby releasing the newly synthesized protein. PrfB is highly specific for a U in the first stop-codon position and recognizes UAA and UGA [pubmed|11158343]

Protein family

• prokaryotic/mitochondrial release factor family (with [protein|59E6468FD891F5271F38543515867B0B4625768D|prfA], according to UniProt)

Structure

• [PDB|1MI6] (from ''E. coli'', 45% identity) [Pubmed|12511960]
• [AF|P28367]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

References

Reviews

• 11158343

Original Publications

• 9882663,20512119,25217634,12511960,33900014