pyrAA

168

carbamoyl-phosphate synthetase (glutaminase subunit)

locus: BSU_15510

Molecular weight: 39.96 kDa

pI: 5.82

Protein length: 364 aa

Gene length: 1095 bp

function: pyrimidine biosynthesis

product: carbamoyl-phosphate synthetase (glutaminase subunit)

essential: no

ec: 6.3.5.5

synonyms: pyrAA

List of homologs in different organisms, belongs to COG0505 (Galperin et al., 2021)

Gene

Coordinates: 1,622,657 → 1,623,751

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The protein

Catalyzed reaction/ biological activity

• 2 ATP + H₂O + hydrogencarbonate + L-glutamine --> 2 ADP + carbamoyl phosphate + 2 H⁺ + L-glutamate + phosphate (according to UniProt)

Protein family

• CarA family (with [protein|4202EAE5D0B2A718382092423DD99E35FDE81218|carA], according to UniProt)

[wiki|Domains]

• [wiki|Glutamine amidotransferase type-1 domain] (aa 171-356) (according to UniProt)

Structure

• [PDB|1JDB] (from ''E. coli'', 47% identity) [Pubmed|10089390]
• [AF|P25993]

Paralogous protein(s)

• [protein|4202EAE5D0B2A718382092423DD99E35FDE81218|carA]

Biological materials

Mutant

• BKE15510 (Δ[gene|E7741ED6F15044900BDC5F0584246469D2D4D586|pyrAA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15510 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GTTTTCCAGTACTAATCGTC,  downstream forward: _UP4_ACAGAGAAAGAAGGGGAAGC
• BKK15510 (Δ[gene|E7741ED6F15044900BDC5F0584246469D2D4D586|pyrAA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15510 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GTTTTCCAGTACTAATCGTC,  downstream forward: _UP4_ACAGAGAAAGAAGGGGAAGC
• GP3716 ([gene|E7741ED6F15044900BDC5F0584246469D2D4D586|pyrAA]::aphA3 trpC2) available in the [wiki|Stülke] lab
• GP3719 ([gene|search|pyrAAAB]::aphA3 trpC2) available in the [wiki|Stülke] lab
• GP3721 ([gene|search|pyrAAAB]::cat trpC2) available in the [wiki|Stülke] lab

Expression vectors

• pGP3772: expression of Strep-''pyrAA'' by [wiki|pGP380] in ''B. subtilis'' suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab

References

Reviews

• 12859215,11395405,11163353

Original Publications

• 8206849,1709162,10089390,8663035,28516784