qoxA

168

cytochrome aa3 quinol oxidase (subunit II)

locus: BSU_38170

Molecular weight: 36.10 kDa

pI: 4.8

Protein length: 321 aa

Gene length: 966 bp

function: respiration

product: cytochrome aa3 quinol oxidase (subunit II)

essential: no

ec: 1.10.3.-

synonyms: qoxA, ipa-37d

List of homologs in different organisms, belongs to COG1622 (Galperin et al., 2021)

Gene

Coordinates: 3,917,296  3,918,261

Phenotypes of a mutant

• inactivation of ''[gene|48A9E1E39838BFFCF12CADF0A8D8E6FFDCA6F175|qoxA]'' restores beta-lactam resistance in a ''[gene|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]'' mutant [Pubmed|22211522]

Advertisement

The protein

Catalyzed reaction/ biological activity

• 2 quinol + O₂ --> 2 quinone + 2 H₂O (according to UniProt)

Protein family

• cytochrome c oxidase subunit 2 family (with [protein|72D5442C0EF3B404F7B615366E357B68C863CEB6|ctaC], according to UniProt)

Structure

• [PDB|1FFT] ([protein|48A9E1E39838BFFCF12CADF0A8D8E6FFDCA6F175|qoxA]-[protein|CBF2DCFFD8195304F942ECBA8596444F0AC47CC6|qoxB]-[protein|CD4520EF427AEFDCF028AE063BC373FA6DCE379E|qoxC] complex from ''E. coli'', 35% identity) [Pubmed|11017202]
• [AF|P34957]

[wiki|Localization]

• cell membrane [Pubmed|18763711]

Additional information

• the [protein|48A9E1E39838BFFCF12CADF0A8D8E6FFDCA6F175|qoxA]-[protein|CBF2DCFFD8195304F942ECBA8596444F0AC47CC6|qoxB]-[protein|CD4520EF427AEFDCF028AE063BC373FA6DCE379E|qoxC]-[protein|03335DC50CED7B38E2380640AE108ADA09A2CBDF|qoxD] cytochrome aa₃ oxidase complex is sensitive to increased intracellular Mn²⁺ concentrations, this results in the generation of toxic reactive radicals [pubmed|34097790]

Expression and Regulation

Operons

• genes: [gene|48A9E1E39838BFFCF12CADF0A8D8E6FFDCA6F175|qoxA]-[gene|CBF2DCFFD8195304F942ECBA8596444F0AC47CC6|qoxB]-[gene|CD4520EF427AEFDCF028AE063BC373FA6DCE379E|qoxC]-[gene|03335DC50CED7B38E2380640AE108ADA09A2CBDF|qoxD]

  description: [Pubmed|1316894]

  regulation

  • [protein|search|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]

  regulatory mechanism

  • stringent response: negative regulation, in [regulon|other_regulator:stringent response|stringent response]
  • [protein|E15E9F88B0E03FE8834C646369D2F4B4E713EF41|citB]: translation control, [pubmed|], in [regulon|protein:E15E9F88B0E03FE8834C646369D2F4B4E713EF41|citB regulon]

  additional information

  • the presence of an iron-responsive element bound by [wiki|CitB] between ''[wiki|feuA]'' and ''[wiki|feuB]'' suggests iron-dependent regulation by [wiki|CitB] [Pubmed|10468622]

Biological materials

Mutant

• BKE38170 ([gene|48A9E1E39838BFFCF12CADF0A8D8E6FFDCA6F175|qoxA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE38170 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACCATCCTTCCTATTGATG,  downstream forward: _UP4_TGATATTGATCAGGAAAGGA
• BKK38170 ([gene|48A9E1E39838BFFCF12CADF0A8D8E6FFDCA6F175|qoxA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK38170 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACCATCCTTCCTATTGATG,  downstream forward: _UP4_TGATATTGATCAGGAAAGGA

References

Reviews

• 33705278

Original Publications

• 36815589,11073895,1316894,20351111,10551842,18763711,24779955,22211522,11017202,27503613,34097790,36422766