queG

168

epoxyqueuosine reductase

locus: BSU_08910

Molecular weight: 48.37 kDa

pI: 7.35

Protein length: 386 aa

Gene length: 1161 bp

function: synthesis of queuosine, tRNA modification

product: epoxyqueuosine reductase

essential: no

synonyms: queG, ygaP, yhbA

List of homologs in different organisms, belongs to COG1600 (Galperin et al., 2021)

Gene

Coordinates: 967,935  969,095

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The protein

Catalyzed reaction/ biological activity

• conversion of epoxyqueuosine to queuosine [Pubmed|21502530]
• AH₂ + epoxyqueuosine³⁴ in tRNA = A + H₂O + queuosine³⁴ in tRNA (according to UniProt)

Protein family

• QueG family (single member, according to UniProt)

[wiki|Domains]

• [wiki|4Fe-4S ferredoxin-type domain] (aa 178-208) (according to UniProt)

[wiki|Cofactors]

• cobalamin
• Fe-S cluster [Pubmed|21502530]

Structure

• [PDB|5D08] [Pubmed|27638883]
• [AF|P97030]

Expression and Regulation

Operons

• genes: [gene|3F22BD8DB7BEC2701B960546AC683603DFCADCCE|queG]

  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-A241 (yhbA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/241 NBRP B. subtilis, Japan]
• BKE08910 ([gene|3F22BD8DB7BEC2701B960546AC683603DFCADCCE|queG]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE08910 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTTTGCACAGCCTCCT,  downstream forward: _UP4_TGACATACGTATAGAGCAGA
• BKK08910 ([gene|3F22BD8DB7BEC2701B960546AC683603DFCADCCE|queG]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK08910 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTTTGCACAGCCTCCT,  downstream forward: _UP4_TGACATACGTATAGAGCAGA

References

• 26230193,21502530,27638883