rnmV

rnmV
168

RNase M5

locus
BSU_00410
Molecular weight
20.53 kDa
pI
6.68
Protein length
Gene length
function
5S ribosomal RNA maturation
product
endoribonuclease M5
essential
no
synonyms
rnmV, yabF

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1658 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
50,087  50,647
Visit Sartorius.com Visit Sartorius.com
Advertisement
The protein
Catalyzed reaction/ biological activity
Endonucleolytic cleavage of RNA, removing 21 and 42 nucleotides, respectively, from the 5'- and 3'-termini of a 5S-rRNA precursor (according to UniProt)
Protein family
Ribonuclease M5 family (single member, according to UniProt)
[wiki|Domains]
N-terminal [wiki|TOPRIM domain] (aa 4-94) [Pubmed|33541205,9722641,16077031]
C-terminal NA-binding domain [pubmed|33541205]
[wiki|Cofactors]
two Mg2+ ions [pubmed|33541205]
Structure
[PDB|6TPQ] (from Geobacillus stearothermophilus, 61% identity) [pubmed|32991829]
[AF|P37547]
[wiki|Localization]
cytoplasm (homogeneous) [Pubmed|16479537]
Expression and Regulation
Operons
genes
[gene|5BA7AA691CFE4635DEFBD0B8B820F487B58A5DB7|rnmV]-[gene|FF329359CBE52C75A39237E8A6BB56F6AB321AFB|ksgA]
description
[Pubmed|11233981]
Open in new tab

[gene|5BA7AA691CFE4635DEFBD0B8B820F487B58A5DB7|rnmV]→[gene|FF329359CBE52C75A39237E8A6BB56F6AB321AFB|ksgA]

2024-02-29 20:40:22

ghost

76

eed4866ffa4f654b46b2cc962cdb9f5b2d8e3935

9FAC2CB8F7E3367C2EFE6DC8D966C339F688D87B

Biological materials
Mutant
MGNA-B906 (yabF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1905 NBRP B. subtilis, Japan]
BKE00410 ([gene|5BA7AA691CFE4635DEFBD0B8B820F487B58A5DB7|rnmV]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00410 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTATTTCCTCCAAGACAA,  downstream forward: _UP4_GATACCGTTATGCGGGAGGA
BKK00410 ([gene|5BA7AA691CFE4635DEFBD0B8B820F487B58A5DB7|rnmV]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00410 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTATTTCCTCCAAGACAA,  downstream forward: _UP4_GATACCGTTATGCGGGAGGA
labs
[wiki|Ciaran Condon], IBPC, Paris, France [http://www.ibpc.fr/UPR9073/condon/index_en.html Homepage]
References
Reviews
31464530,35787606
Original Publications
16479537,11233981,11976317,16077031,9722641,32991829,33541205,32991829

5BA7AA691CFE4635DEFBD0B8B820F487B58A5DB7

Page visits: 2905

Time of last update: 2024-03-28 11:09:45

Author of last update: Jstuelk