rodZ

rodZ
168

required for proper septum positioning during vegetative growth ([wiki|cell shape] determination), part of the [protein|search|Rod complex] for lateral [wiki|cell wall synthesis] and control of cell diameter

locus
BSU_16910
Molecular weight
23.48 kDa
pI
4.68
Protein length
Gene length
function
septum positioning during vegetative growth
product
morphogenic protein
essential
yes
synonyms
rodZ, ymfM

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1426 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,761,707  1,762,573
Phenotypes of a mutant
essential [Pubmed|19164570,23879732]
depletion results in the formation of shorter and rounder cells [Pubmed|23879732]
depletion results in disturbed formation of asymmetric septa and perturbed [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF] activation [Pubmed|27415800]
hypersensitivity to sorbic acid stress [Pubmed|27818647]
small, round, DNA-containing cells [pubmed|29403445]
the mutant cells are thicker and longer than wild type cells [pubmed|34846166]
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The protein
Catalyzed reaction/ biological activity
the presence of [protein|6071A84EF190C820DB8985C08ABC74F744B793DB|rodZ]-P results in increased [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB] filament density and growth rate [pubmed|36797487]
[wiki|Domains]
N-terminal cytoplasmic domain (RodZn), a transmembrane domain, and C-terminal extra-cytoplasmic domain (RodZc) [Pubmed|25503291]
Structure
[PDB|3FYM] (from Staphylococcus aureus, corresponds to aa 1 ... 112, 31% identity)
[AF|O31771]
Modification
phosphorylation by [protein|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|prkC] in response to lipid II abundance [pubmed|36797487]
[wiki|Localization]
membrane protein (according to UniProt)
Additional information
overexpression results in growth inhibition in minimal medium (in the absence of Mg2+ ) [Pubmed|19164570]
RodZ is destabilized in a ''[gene|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB]'' mutant [Pubmed|23879732]
Expression and Regulation
Operons
genes
[gene|725094DC9353EBB5FD603AB389607D8C13DF5F51|ymfK/1]-[gene|9394EA0675DFFCB5F0ECD494C104A66CE13A8EF6|ymfK/2]-[gene|6071A84EF190C820DB8985C08ABC74F744B793DB|rodZ]
description
[Pubmed|22383849]
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[gene|725094DC9353EBB5FD603AB389607D8C13DF5F51|ymfK/1]→[gene|6071A84EF190C820DB8985C08ABC74F744B793DB|rodZ]

2024-02-26 02:57:24

Jstuelk

58

b950e91cac4165ef381d23ef9a8e7e69ea47bc84

11B94A37223A14387217043C4CD3317EAFFE5485

Biological materials
Mutant
MGNA-B120 (ymfM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1119 NBRP B. subtilis, Japan]
BKE16910 ([gene|6071A84EF190C820DB8985C08ABC74F744B793DB|rodZ]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE16910 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTGCTTTTTCCTCTCTGG,  downstream forward: _UP4_TAATTACCAGATGACTTTTC
BKK16910 ([gene|6071A84EF190C820DB8985C08ABC74F744B793DB|rodZ]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK16910 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTGCTTTTTCCTCTCTGG,  downstream forward: _UP4_TAATTACCAGATGACTTTTC
labs
[wiki|Adriano Henriques], Lisbon, Portugal  [http://www.itqb.unl.pt/research/biology/microbial-development homepage]
References
Reviews
33709487
Original Publications
36815589,36797487,19164570,21636744,21636745,23879732,25503291,27415800,27818647,29403445,30651563,31086310

6071A84EF190C820DB8985C08ABC74F744B793DB

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Time of last update: 2024-03-29 09:51:54

Author of last update: Jstuelk