rpmA

168

ribosomal protein bL27

locus: BSU_27940

Molecular weight: 10.23 kDa

pI: 10.76

Protein length: 94 aa

Gene length: 285 bp

function: translation

product: ribosomal protein L27 (bL27)

essential: yes

synonyms: rpmA

List of homologs in different organisms, belongs to COG0211 (Galperin et al., 2021)

Gene

Coordinates: 2,854,880  2,855,164

Phenotypes of a mutant

• essential [Pubmed|23002217,12682299]

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The protein

Protein family

• bacterial [wiki|ribosomal protein] bL27 family (single member, according to UniProt)

Structure

• [PDB|3J9W] (the [wiki|ribosome]) [Pubmed|25903689]
• [AF|P05657]

Modification

• the N-terminal extension is cleaved off by [protein|B27548A4BBF396C9BA102A80ACB08B21262CEA30|prp] [Pubmed|25388641]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Additional information

• the protein is significantly underrepresented in 45S assembly intermediates that accumulate upon depletion of [protein|54CD247926F7FABE4ACE85EB7021B62500B47260|rbgA] [Pubmed|24335279]

Expression and Regulation

Operons

• genes: [gene|11927EB0448839D047EC7BF70EC65156DAB481D3|rplU]-[gene|B27548A4BBF396C9BA102A80ACB08B21262CEA30|prp]-[gene|4626C659D6F1A81CCC94030F1CBAF7FEC3DE19CA|rpmA]

  description: [Pubmed|22383849]

  regulation

  • [protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|rel] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
  • likely autorepression of the [gene|11927EB0448839D047EC7BF70EC65156DAB481D3|rplU]→[gene|4626C659D6F1A81CCC94030F1CBAF7FEC3DE19CA|rpmA] operon expression upon binding of excess of one ribosomal protein to the untranslated region of the [gene|11927EB0448839D047EC7BF70EC65156DAB481D3|rplU]→[gene|4626C659D6F1A81CCC94030F1CBAF7FEC3DE19CA|rpmA] mRNA [Pubmed|17616982]

  regulatory mechanism

  • stringent response: negative regulation, in [regulon|other_regulator:stringent response|stringent response]

• genes: [gene|AB2A3422277040107AAF90358BBE58608F8E0738|spoIVFA]-[gene|8801B095C2CE36F3E0B9DBBF489FFB217087DC0A|spoIVFB]-[gene|11927EB0448839D047EC7BF70EC65156DAB481D3|rplU]-[gene|B27548A4BBF396C9BA102A80ACB08B21262CEA30|prp]-[gene|4626C659D6F1A81CCC94030F1CBAF7FEC3DE19CA|rpmA]

  description: [Pubmed|22383849]

  regulation

  • expressed early during sporulation in the mother cell ([protein|search|SigE], [wiki|SpoIIID]) [Pubmed|15699190,1942049,15383836]

  additional information

  • the domains that inhibits [protein|search|SpoIVFB] is cleaved off by [protein|search|CtpB] (second cleavage) [PubMed|24243021]

• genes: [gene|4626C659D6F1A81CCC94030F1CBAF7FEC3DE19CA|rpmA]

  description: [Pubmed|8969504]

References

• 36815589,19653700,8969504,23002217,24335279,25388641,22383849,25903689,28516784