rpmGA

168

ribosomal protein bL33a

locus: BSU_24900

Molecular weight: 5.85 kDa

pI: 10.44

Protein length: 49 aa

Gene length: 150 bp

function: translation

product: ribosomal protein L33a (bL33a)

essential: no

synonyms: rpmGA

List of homologs in different organisms, belongs to COG0267 (Galperin et al., 2021)

Gene

Coordinates: 2,574,408 → 2,574,557

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The protein

Protein family

• bacterial [wiki|ribosomal protein] bL33 family (with [protein|BBD82B93D5E880AB7093ED53DDB149B0E0B3BAE4|rpmGB] and [protein|5189DF2F6144CD2429B5C01B1AD03BAE98307579|rpmGC], according to UniProt)

[wiki|Cofactors]

• requires zinc for activity [Pubmed|19648245]

Structure

• [PDB|3J9W] (the [wiki|ribosome]) [Pubmed|25903689]
• [AF|P56849]

Modification

• phosphorylated on Arg-29 [Pubmed|22517742]

Paralogous protein(s)

• [protein|BBD82B93D5E880AB7093ED53DDB149B0E0B3BAE4|rpmGB], [protein|5189DF2F6144CD2429B5C01B1AD03BAE98307579|rpmGC]

[wiki|Localization]

• [wiki|ribosome] (according to UniProt)

Additional information

• the protein is significantly underrepresented in 45S assembly intermediates that accumulate upon depletion of [protein|54CD247926F7FABE4ACE85EB7021B62500B47260|rbgA] [Pubmed|24335279,23700310]

Expression and Regulation

Operons

• genes: [gene|D73FC9CAD929919050837824B4794F9FC9E83052|rpmGA]

  description: [pubmed|22383849]

  regulation

  • expression is reduced in motile cells as compared to non-motile cells [pubmed|33782055]

• genes: [gene|402CD558623D1D520D3401F03CF021F42B3894A9|yqgL]-[gene|52BD8C536202E6D0B2B1F13E677CB2534F80BCEF|yqgM]-[gene|D73FC9CAD929919050837824B4794F9FC9E83052|rpmGA]

  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE24900 (Δ[gene|D73FC9CAD929919050837824B4794F9FC9E83052|rpmGA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24900 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTATTTTCCCTCCAAACT,  downstream forward: _UP4_TAACAGCTTTGCTGTTTGAG
• BKK24900 (Δ[gene|D73FC9CAD929919050837824B4794F9FC9E83052|rpmGA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24900 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTATTTTCCCTCCAAACT,  downstream forward: _UP4_TAACAGCTTTGCTGTTTGAG

References

• 19648245,22517742,23002217,24335279,23700310,25903689,33753464,33782055