rpsD

168

ribosomal protein uS4

locus: BSU_29660

Molecular weight: 22.69 kDa

pI: 10.24

Protein length: 200 aa

Gene length: 603 bp

function: translation

product: ribosomal protein S4 (uS4)

essential: yes

synonyms: rpsD

List of homologs in different organisms, belongs to COG0522 (Galperin et al., 2021)

Gene

Coordinates: 3,035,730  3,036,332

Phenotypes of a mutant

• essential [Pubmed|23002217,12682299]

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The protein

Protein family

• [wiki|S4 RNA-binding domain] superfamily (according to http://www.ebi.ac.uk/interpro/entry/IPR036986 Interpro)
• universal [wiki|ribosomal protein] uS4 family (single member, according to UniProt)

[wiki|Domains]

• [wiki|S4 RNA-binding domain] (aa 92-155) (according to UniProt)

Structure

• [PDB|1C06] (Geobacillus stearothermophilus)
• [PDB|3J9W] (the [wiki|ribosome]) [Pubmed|25903689]
• [AF|P21466]

Expression and Regulation

Operons

• genes: [gene|789D08564463542B8D96FC12F408549A669703CE|rpsD]

  description: [Pubmed|1699930]

  regulation

  • autorepression of ''rpsD'' expression upon binding of excess [protein|789D08564463542B8D96FC12F408549A669703CE|rpsD] to a [wiki|RNA switch] in the untranslated region of the ''rpsD'' mRNA [Pubmed|27120414,23611891,1906866]
  • expression is reduced in motile cells as compared to non-motile cells [pubmed|33782055]

  regulatory mechanism

  • stringent response: negative regulation, in [regulon|other_regulator:stringent response|stringent response]
  • [protein|789D08564463542B8D96FC12F408549A669703CE|rpsD]: autorepression, [pubmed|27120414,23611891,1906866], in [regulon|protein:789D08564463542B8D96FC12F408549A669703CE|rpsD regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|1699930], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

  additional information

  • term-seq has identified a potential novel regulatory RNA element including an intrinsic transcription terminator upstream of ''rpsD'' [Pubmed|27120414]
  • the [wiki|RNA switch] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]

References

• 36815589,1699930,2121712,6420647,1400226,1906866,19653700,11489846,23611891,23002217,25548247,25548248,26115952,25903689,27120414,29794222,33782055