rpsT

168

ribosomal protein bS20

locus: BSU_25550

Molecular weight: 9.36 kDa

pI: 11.24

Protein length: 88 aa

Gene length: 267 bp

function: translation

product: ribosomal protein S20 (bS20)

essential: no

synonyms: rpsT, yqeO

List of homologs in different organisms, belongs to COG0268 (Galperin et al., 2021)

Gene

Coordinates: 2,635,815  2,636,081

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The protein

Protein family

• bacterial [wiki|ribosomal protein] bS20 family (single member, according to UniProt)

Structure

• [PDB|3J9W] (the [wiki|ribosome]) [Pubmed|25903689]
• [AF|P21477]

Additional information

• this ribosomal protein is lacking in some organisms with very small genomes [pubmed|33753464]

Expression and Regulation

Operons

• genes: [gene|697B714A2AC7B418A7F699B4547BE896B3A8A28B|rpsT]

  description: [pubmed|22383849]

  regulation

  • expression is reduced in motile cells as compared to non-motile cells [pubmed|33782055]

Biological materials

Mutant

• BKE25550 ([gene|697B714A2AC7B418A7F699B4547BE896B3A8A28B|rpsT]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE25550 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTGTTTCACCTCCTAAG,  downstream forward: _UP4_TCTGCATAATATAAAAACGA
• BKK25550 ([gene|697B714A2AC7B418A7F699B4547BE896B3A8A28B|rpsT]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK25550 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTGTTTCACCTCCTAAG,  downstream forward: _UP4_TCTGCATAATATAAAAACGA

References

• 36815589,19653700,23002217,25903689,33753464,33782055