rsbS

168

part of the [wiki|stressosome], control of [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB] activity

locus: BSU_04680

Molecular weight: 13.17 kDa

pI: 4.14

Protein length: 121 aa

Gene length: 366 bp

function: control of [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB] activity

product: scaffold protein of the stressosome, anti-[protein|ADC52A22950736A0435AEEEC43F7407878786A81|rsbT]

essential: no

synonyms: rsbS, ycxS

List of homologs in different organisms, belongs to COG1366 (Galperin et al., 2021)

Gene

Coordinates: 520,237  520,602

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The protein

[wiki|Domains]

• [wiki|STAS domain] (aa 4-115) (according to UniProt)

Structure

• [PDB|3VY9] (complete stressosome)
• [PDB|6JHK]
• [AF|P42410]

Modification

• phosphorylation on Ser-59 by [protein|ADC52A22950736A0435AEEEC43F7407878786A81|rsbT] [Pubmed|17218307], dephosphorylation by [protein|8536803BDFB58D43C52961931F609933E49E989D|rsbX] [Pubmed|21362065]

Expression and Regulation

Operons

• genes: [gene|621357D1FB6AC1B0499732EFE226F982B26CE34E|rsbR]-[gene|841CE7FCA4E84445830CA18F9856F6F30014E3BB|rsbS]-[gene|ADC52A22950736A0435AEEEC43F7407878786A81|rsbT]-[gene|0B8381D2C724DAA724E4AFC67000F12AD112D13D|rsbU]-[gene|AC64DA463250A090A62E50901EFE653C8F963872|rsbV]-[gene|5AF5F199C5D92E13DE1C56E28948A557E3954CBF|rsbW]-[gene|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]-[gene|8536803BDFB58D43C52961931F609933E49E989D|rsbX]

  description: [Pubmed|8002610]

  regulation

  • ''[protein|search|rsbV]:'' induced by stress ([protein|search|SigB]) [Pubmed|11544224]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|20454630], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8002610], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|11544224], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]

Biological materials

Mutant

• BKE04680 ([gene|841CE7FCA4E84445830CA18F9856F6F30014E3BB|rsbS]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE04680 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CGGGATTTTCGGATGTCTCA,  downstream forward: _UP4_AAGCGGGAATTGGGGGAATA
• BKK04680 ([gene|841CE7FCA4E84445830CA18F9856F6F30014E3BB|rsbS]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK04680 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CGGGATTTTCGGATGTCTCA,  downstream forward: _UP4_AAGCGGGAATTGGGGGAATA

labs

• [wiki|Bill Haldenwang], San Antonio, USA
• [wiki|Chet Price], Davis, USA [http://foodscience.ucdavis.edu/price_lab/pricelab.html homepage]

References

Reviews

• 19704888,16319496,20658979,28271471,33042030

Original Publications

• 36815589,8002610,8682769,9786195,8682789,10781545,15583165,8824586,10329124,17158665,21821766,16321960,10671474,8808936,15312768,11244072,15342582,16321960,8955331,12950928,15466036,9179850,18832644,17218307,20019076,24599254,23320651,21362065,28727759,28727759