secA

168

preprotein translocase subunit (ATPase), required for membrane targeting of DivIVA, motor protein that drives preprotein translocation through the SecY-SecE-SecG channel

locus: BSU_35300

Molecular weight: 95.33 kDa

pI: 5.34

Protein length: 841 aa

Gene length: 2526 bp

function: protein secretion

product: preprotein translocase subunit (ATPase)

essential: yes

synonyms: secA, div, div-341, ts-341

List of homologs in different organisms, belongs to COG0653 (Galperin et al., 2021)

Gene

Coordinates: 3,628,310  3,630,835

Phenotypes of a mutant

• essential [Pubmed|12682299]
• terminal phenotype of CRISPRi knockdown mutant is moderate bending and a cessation of growth [Pubmed|27238023]

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The protein

Catalyzed reaction/ biological activity

• ATP  ADP + Pi + preprotein translocation
• required for membrane targeting of [protein|A8C0C2B7BCA6FFFF3811402D683FB7B99F7120A4|divIVA] [Pubmed|24592260]

Protein family

• SecA family (single member, according to UniProt)

[wiki|Domains]

• nucleotide binding domain, preprotein binding domain, IRA2 domain, scaffold domain, wing domain, IRA1 domain, C-terminal domain

[wiki|Cofactors]

• Mg

Structure

• [PDB|1TF5] (open structure),  [PDB|2IBM], [PDB|3DL8] (structure of the ([protein|5350DC618130E013757EE667B9AFC5F2EC68EC08|secY]-[protein|1007EA15FB7AF81A44400287DB68BCC497158ACF|secE]-[protein|D8851F453E5EF18D36F25834A8CB9C61EAF15E44|secG])-[protein|AD7CD451B4AE638A719C91780339784A7FF0F248|secA] complex [Pubmed|18923516]
• [AF|P28366]

Effectors of protein activity

• anionic phospholipids, preprotein, [protein|5350DC618130E013757EE667B9AFC5F2EC68EC08|secY], signal peptides (even when added in trans) [Pubmed|19924216]

[wiki|Localization]

• mainly in the cytoplasm [pubmed|38474341]
• a small fraction is statically associated with the cell membrane [pubmed|38474341]

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]

Expression and Regulation

Operons

• genes: [gene|AD7CD451B4AE638A719C91780339784A7FF0F248|secA]-[gene|12E06FD3A36D95AE82E3C65788FA98178039592F|prfB]

  description: [Pubmed|9882663]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|9882663], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• BEC35300, a CRISPRi knockdown mutant, is available from the [http://bgsc.org/ Bacillus Genetic Stock Center]

References

Reviews

• 25212246,27890920,27799326

Original Publications

• 19933328,19924216,18923516,12873133,12242434,1385592,10816431,19850053,9880811,12218047,17981983,9882663,23484952,20574771,23167435,23794293,23852076,24592260,24786965,11021932,7851746,7894702,10074074,8497195,8440733,7706219,7642557,27336478,27238023,27795329,28041832,27802584,22354575,38474341