sipS

168

signal peptidase I

locus: BSU_23310

Molecular weight: 20.90 kDa

pI: 9.72

Protein length: 184 aa

Gene length: 555 bp

function: protein secretion

product: signal peptidase I

essential: no

ec: 3.4.21.89

synonyms: sipS

List of homologs in different organisms, belongs to COG0681 (Galperin et al., 2021)

Gene

Coordinates: 2,432,316 → 2,432,870

Phenotypes of a mutant

• a ''[gene|CDC6971F39F3EEAAE912CB1402EE1BD64D5A12A0|sipS] [gene|C2EAE417DB9250B88BB0E25D4AE760BDE82EFD86|sipT]'' double mutant is not viable [Pubmed|9694797]

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The protein

Catalyzed reaction/ biological activity

• Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins (according to UniProt)
• intra-membrane cleavage and thus inactivation of [protein|ED1DDA187692683D9862EC0E858B80CD56C48045|rsiV] between A66 and M67 [Pubmed|25275625]

Protein family

• [wiki|peptidase S26 family] (according to UniProt)

Structure

• [PDB|4NV4] (from ''Bacillus anthracis'', 40% identity)
• [AF|P28628]

Paralogous protein(s)

• [protein|9824F9CF9C2028AF267AC43FE7FD8590E8231AB8|sipU], [protein|ADEEA5E15C100C1DC6E129B9A3E6DECAFCA8C409|sipV], [protein|C2EAE417DB9250B88BB0E25D4AE760BDE82EFD86|sipT]

[wiki|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|9756DD153F0FAACB5A64B363D580AA8A9AE7FD58|ypzJ]-[gene|CDC6971F39F3EEAAE912CB1402EE1BD64D5A12A0|sipS]

  description: [Pubmed|22383849]

• genes: [gene|CDC6971F39F3EEAAE912CB1402EE1BD64D5A12A0|sipS]

  description: [Pubmed|8951809]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8951809], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• BKE23310 (Δ[gene|CDC6971F39F3EEAAE912CB1402EE1BD64D5A12A0|sipS]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23310 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAACTGATTTCCTCCAATAA,  downstream forward: _UP4_TAGGATCAAGCAGCTTCCCA
• BKK23310 (Δ[gene|CDC6971F39F3EEAAE912CB1402EE1BD64D5A12A0|sipS]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23310 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAACTGATTTCCTCCAATAA,  downstream forward: _UP4_TAGGATCAAGCAGCTTCCCA

labs

• [wiki|Jan Maarten van Dijl], Groningen, Netherlands

References

Reviews

• 22688815,31286585,34410368

Original Publications

• 9325333,9694797,8951809,10336490,25275625,28088521,29358498