spoIIT
168
co-activator (with [protein|9F338225E1286D530EAAAB504DEC8D5AFA06AC8D|spoIIR]) for triggering [protein|221829E084DA78ACE1C2DFCEB6E59EDA534FF55D|spoIIGA]-dependent processing of [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]
locus
BSU_36770
Molecular weight
28.48 kDa
pI
6.88
function
control of [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE] processing and activation
product
co-activator (with [protein|9F338225E1286D530EAAAB504DEC8D5AFA06AC8D|spoIIR]) for triggering [protein|221829E084DA78ACE1C2DFCEB6E59EDA534FF55D|spoIIGA]-dependent processing of [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]
essential
no
synonyms
spoIIT, ywmB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms
This gene is a member of the following regulons
Gene
Coordinates
3,779,293 3,780,033
Phenotypes of a mutant
mild sporulation defect due to inefficient activation of [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE] [Pubmed|26735940]
inactivation of ''[gene|3A8E0417C4EC85FDDE1178B775F6579C8F1009C6|spoIIT]'' reduces sporulation efficiency to 38% that of wild type cells; abortively disporic [Pubmed|26735940]
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The protein
Structure
[PDB|2FPN]
[AF|O32277]
[wiki|Localization]
secreted from the forespore to the intermembrane space between the forespore and the mother cell [Pubmed|26735940]
Expression and Regulation
Operons
genes
[gene|32CE4F77F08F926152BECAB56E2841E1FFB27600|ywzB]-[gene|3A8E0417C4EC85FDDE1178B775F6579C8F1009C6|spoIIT]-[gene|DA763F7C2192655C590752C2A285ADF902B11BCA|murAA]
description
[Pubmed|22383849]
regulation
expressed early during sporulation in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]) [Pubmed|26735940]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|22383849], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]: sigma factor, [Pubmed|26735940], in [regulon|protein:CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF regulon]
genes
[gene|3A8E0417C4EC85FDDE1178B775F6579C8F1009C6|spoIIT]-[gene|DA763F7C2192655C590752C2A285ADF902B11BCA|murAA]
description
[Pubmed|26735940]
regulation
expressed early during sporulation in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]) [Pubmed|26735940]
Biological materials
Mutant
MGNA-A189 (ywmB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/189 NBRP B. subtilis, Japan]
BKE36770 ([gene|3A8E0417C4EC85FDDE1178B775F6579C8F1009C6|spoIIT]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE36770 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTCATCTCTCCCCCTT, downstream forward: _UP4_TAATATAGAGAATTTAGGGA
BKK36770 ([gene|3A8E0417C4EC85FDDE1178B775F6579C8F1009C6|spoIIT]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK36770 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTCATCTCTCCCCCTT, downstream forward: _UP4_TAATATAGAGAATTTAGGGA
References
Reviews
Original Publications
Page visits: 1891
Time of last update: 2024-04-20 16:33:33
Author of last update: Jstuelk