spoIVA

168

ATPase, spore coat morphogenetic protein, anchors the spore coat to the spore surface via [protein|BBDA32A4EE3389D6F4404F7B3DA9E13AC7BF8055|spoVM]

locus: BSU_22800

Molecular weight: 55.01 kDa

pI: 4.55

Protein length: 492 aa

Gene length: 1479 bp

function: initiation of spore coat assembly

product: ATPase, basement layer protein for spore coat assembly

essential: no

synonyms: spoIVA, spoVP

List of homologs in different organisms, belongs to COG5831 (Galperin et al., 2021)

Gene

Coordinates: 2,386,195  2,387,673

Phenotypes of a mutant

• the spore coat does not localize to the spore surface but self-assembles into aggregates in the mother cell cytoplasm [Pubmed|22171814]

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The protein

Catalyzed reaction/ biological activity

• uses ATP hydrolysis to drive self-assembly into static filaments [Pubmed|23267091]
• ATP hydrolysis drives polymerization of a nucleotide-free filament [Pubmed|23267091]
• ploymerization depends on a critical threshold concentration of SpoIVA that is only achieved once the protein is recruited to the surface of the developing spore [Pubmed|23267091]
• ATP + H₂O --> ADP + H⁺ + phosphate (according to UniProt)

[wiki|Domains]

• contains a Walker A ATPase domain

Structure

• [AF|P35149]

Modification

• phosphorylated on Arg-454 [pubmed|31221751]

[wiki|Localization]

• innermost protein of the spore coat basement [Pubmed|22171814]

Expression and Regulation

Operons

• genes: [gene|A25C1530DA7BB007A288E525404E9F775E219FE8|spoIVA]

  description: [Pubmed|1729246]

  regulation

  • expressed early during sporulation in the mother cell ([protein|search|SigE], [wiki|SpoIIID]) [Pubmed|1729246,15699190,15383836]

  regulatory mechanism

  • [protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID]: repression, [Pubmed|15383836], in [regulon|protein:90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID regulon]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|1729246,15699190], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]

Biological materials

Mutant

• BKE22800 ([gene|A25C1530DA7BB007A288E525404E9F775E219FE8|spoIVA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22800 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAGTGATCCCCTCCCGGAC,  downstream forward: _UP4_TAATACCGGTAGACCTCTTT
• BKK22800 ([gene|A25C1530DA7BB007A288E525404E9F775E219FE8|spoIVA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22800 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAGTGATCCCCTCCCGGAC,  downstream forward: _UP4_TAATACCGGTAGACCTCTTT

References

Reviews

• 22192522,23202530,26418292,28408070,29355854,34438398,35081523,35638784

Original Publications

• 8748030,15699190,1729246,8936302,1729247,9922240,7592342,8299942,1691789,12644503,18691972,17427285,19775244,22171814,15383836,23267091,19702880,22262582,24810258,25854653,26387458,31221751,31597735,33573199,33704064,38141557