thiC

168

4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate (HMP-P) synthase, biosynthesis of the pyrimidine moiety of thiamine

locus: BSU_08790

Molecular weight: 65.74 kDa

pI: 5.26

Protein length: 590 aa

Gene length: 1773 bp

function: biosynthesis of thiamine

product: 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate (HMP-P) synthase

essential: no

synonyms: thiC, thiA

List of homologs in different organisms, belongs to COG0422 (Galperin et al., 2021)

Gene

Coordinates: 955,895  957,667

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The protein

Catalyzed reaction/ biological activity

• 5-amino-1-(5-phospho-β-D-ribosyl)imidazole + S-adenosyl-L-methionine --> 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + 5'-deoxyadenosine + CO + formate + 3 H⁺ + L-methionine (according to UniProt)

Protein family

• thiC family (single member, according to UniProt)

[wiki|Cofactors]

• Fe-S cluster [pubmed|29292548]

Structure

• [PDB|3EPN] (from Caulobacter crescentus, 65% identity) [pubmed|18953358]
• [AF|P45740]

Modification

• phosphorylated on Arg-556 [Pubmed|22517742]
• phosphorylated on Ser-565, Ser-586 and Tyr-589 [Pubmed|20509597]

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation [Pubmed|17981983]

Expression and Regulation

Operons

• genes: [gene|51B5F268EE71DD28741A38F72E507B6E245D5494|thiC]

  description: [Pubmed|22383849]

  regulation

  • repressed by thiamine ([wiki|Thi-box]) [Pubmed|12376536]
  • the [wiki|Thi-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]

  regulatory mechanism

  • [protein|E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box]: RNA switch, in [regulon|protein:E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box regulon]

Biological materials

Mutant

• 1A603 ( ''thiC''::''erm''), [Pubmed|3015878], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A603&Search=1A603 BGSC]
• BKE08790 ([gene|51B5F268EE71DD28741A38F72E507B6E245D5494|thiC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE08790 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTACTTTTCCTTCCCCTT,  downstream forward: _UP4_TAAAAAAAATGAAGATGGAG
• BKK08790 ([gene|51B5F268EE71DD28741A38F72E507B6E245D5494|thiC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK08790 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTACTTTTCCTTCCCCTT,  downstream forward: _UP4_TAAAAAAAATGAAGATGGAG

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab

References

Reviews

• 19348578,10382260

Original Publications

• 16291685,9370266,20509597,12376536,22517742,22383849,18953358,28516784,29794222