tuaD

168

UDP glucose 6-dehydrogenase

locus: BSU_35580

Molecular weight: 49.65 kDa

pI: 6.11

Protein length: 461 aa

Gene length: 1386 bp

function: biosynthesis of teichuronic acid

product: UDP glucose 6-dehydrogenase

essential: no

ec: 1.1.1.22

synonyms: tuaD, yvhD

List of homologs in different organisms, belongs to COG1004 (Galperin et al., 2021)

Gene

Coordinates: 3,654,139 → 3,655,524

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The protein

Catalyzed reaction/ biological activity

• H₂O + 2 NAD⁺ + UDP-α-D-glucose --> 3 H⁺ + 2 NADH + UDP-α-D-glucuronate (according to UniProt)

Protein family

• UDP-glucose/GDP-mannose dehydrogenase family (with [protein|59024C05AA0106719C81E45E3B060A6F710B2690|ytcA] and [protein|45AFD434F5262BFBE4ED6DF3D18DAED61FF317D1|ugd], according to UniProt)

Structure

• [PDB|3GG2] (from Porphyromonas gingivalis, 48% identity)
• [AF|O32271]

Modification

• phosphorylation on a Tyr residue by [protein|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|ptkA] [Pubmed|12970183], dephosphorylated by [protein|CF73D86DB024575BE8F043A4C3996458F4262E46|ptpZ] [Pubmed|15866923]

Effectors of protein activity

• [protein|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|ptkA]-dependent phosphorylation stimulates TuaD activity [Pubmed|12970183]

Paralogous protein(s)

• [protein|45AFD434F5262BFBE4ED6DF3D18DAED61FF317D1|ugd], [protein|59024C05AA0106719C81E45E3B060A6F710B2690|ytcA]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Biological materials

Mutant

• BKE35580 (Δ[gene|DF8CD0B3997A7A4CF4821A7D64E9898422FD17E0|tuaD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE35580 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACAGCGCTCAACCCTCTCC,  downstream forward: _UP4_TAAAAAGAAAAAGTGTTACT
• BKK35580 (Δ[gene|DF8CD0B3997A7A4CF4821A7D64E9898422FD17E0|tuaD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK35580 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACAGCGCTCAACCCTCTCC,  downstream forward: _UP4_TAAAAAGAAAAAGTGTTACT

References

• 10913081,10376820,9611818,12970183,10048024,10627039,15866923,26923784