walR

walR
168

two-component response regulator ([wiki|OmpR family]), controls cell wall metabolism

locus
BSU_40410
Molecular weight
27.08 kDa
pI
4.88
Protein length
Gene length
function
control of cell wall metabolism
product
two-component response regulator ([wiki|OmpR family])
essential
yes
synonyms
walR, yycF

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0745 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
4,153,696  4,154,403
Phenotypes of a mutant
essential [Pubmed|12682299]
essentiality can be bypassed by constitutive expression of [gene|321F248C22D7283C0F3323F1F4069E36F8D7FE6C|lytE] or [gene|4F9D70C4BB4FCA809BEAC341C3F8122FB7FD8872|cwlO] in a [gene|603E226CE488A25C4E28A6A7363CCD65BE64BB21|pdaC] [gene|0CA55371306D4BD768CFA82027DCB4D581BCCB87|iseA] mutant background [pubmed|29465029]
a constitutively active form of [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR] ([protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR]-R204C) results in constitutive expression of the [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR] regulon, and in combination with a ''[gene|DB09F1C36257F511A84A083967A25A9D46744D14|sepF]'' mutation to the formation of cell wall-less L-forms  [Pubmed|22122227]
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The protein
Protein family
[wiki|OmpR family] of two-component response regulators
[wiki|Domains]
[wiki|Response regulatory domain] (aa 4-117) (according to UniProt)
Structure
[PDB|2OQR] (from Mycobacterium tuberculosis, 48% identity) [pubmed|17942407]
[PDB|2D1V] (DNA binding domain)[PDB|2ZWM] (receiver domain)
[AF|P37478]
Modification
phosphorylated by [protein|E88978809272FAC2520DB4ABCA0554F8028F3451|walK] on Asp-53
phosphorylated by [protein|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|prkC] on Thr-101, this stimulates [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR] activity [Pubmed|26102633]
Effectors of protein activity
phosphorylation likely affects DNA-binding activity
Paralogous protein(s)
[protein|6A37531896205A9B894C81AB0563C216C0B52CD7|ykoG], [protein|706983E6942E883D3A9D45693E7B4015AEABE60B|yclJ], [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR]-[gene|E88978809272FAC2520DB4ABCA0554F8028F3451|walK]-[gene|06D2D03C65D50E3909B2C93835193815B6016967|walH]-[gene|EE681C9A42FCC29CBB55CC9566EE4CFF986D0F1D|walI]-[gene|D5321F707E183E72B225F1CED2F2FDCB1183B88A|walJ]-[gene|75B8C37E589C855D2BAD7FB51B9600E161ACFBD3|htrC]
description
[Pubmed|9829949]
regulation
''htrC'' transcript expressed during sporulation [Pubmed|9829949]
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[gene|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR]→[gene|75B8C37E589C855D2BAD7FB51B9600E161ACFBD3|htrC]

2024-03-24 13:36:40

ghost

104

4e891bb3dceb8eda2e5ad4f9ed646a611afbe349

32BB8D419E54714C8925B07FBBCF691251CA4145

Biological materials
Mutant
MGNA-B828 (yycF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1827 NBRP B. subtilis, Japan]
Expression vectors
pGP2633, constitutive expression in ''B. subtilis'' (based on [wiki|pBQ200]), available in [wiki|Jörg Stülke]'s lab
pGP2635, expression of His-[protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR] in ''E. coli'' (based on [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
labs
[wiki|Kevin Devine], Dublin
References
Reviews
19019149,18245295,18792692,22126136,10094672,28886686
Original Publications
18573169,17350627,10094672,17827301,9829949,16030236,20111065,20167622,20834167,24125693,22122227,24125693,24490131,18789936,26102633,17942407,29465029,31339578,31723135,34910349
The [wiki|WalR regulon]
17581128,10878122,12950927,23199363

7F340423A34CE40D1F1AA8D373F7C4B859A6496D

Page visits: 8234

Time of last update: 2024-03-29 00:22:14

Author of last update: Jstuelk