abf2

168

alpha-L-arabinofuranosidase

locus: BSU_28510

Molecular weight: 56.34 kDa

pI: 5.33

Protein length: 495 aa

Gene length: 1488 bp

function: arabinan degradation

product: alpha-L-arabinofuranosidase

essential: no

ec: 3.2.1.55

synonyms: abf2, xsa

List of homologs in different organisms, belongs to COG3534 (Galperin et al., 2021)

Gene

Coordinates: 2,913,661  2,915,148

Advertisement

The protein

Catalyzed reaction/ biological activity

• Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides (according to UniProt)

Protein family

• glycosyl hydrolase 51 family (with [protein|2B2629D275491A9F7031D5A99BAA001B011A765B|abfA], according to UniProt)

Structure

• [PDB|2VRQ] (from Thermobacillus xylanilyticus, 66% identity) [pubmed|18563919]
• [AF|P94552]

Expression and Regulation

Operons

• genes: [gene|1DF08A474E95909EAF68CF26D97DD49350ABEA89|abf2]

  description: [Pubmed|14973026]

  regulation

  • induced by arabinose ([protein|search|AraR]) [Pubmed|14973026]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|22900538], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
  • [protein|A567466894AE9DE7CDE7816615433A37532297B5|araR]: repression, [Pubmed|14973026], in [regulon|protein:A567466894AE9DE7CDE7816615433A37532297B5|araR regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|14973026], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

  additional information

  • A [protein|search|ncRNA] is predicted between '[protein|search|trxA]' and '[protein|search|abf2]' [PubMed|20525796]

Biological materials

Mutant

• BKE28510 ([gene|1DF08A474E95909EAF68CF26D97DD49350ABEA89|abf2]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE28510 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGCCTCTGATCATTCCTT,  downstream forward: _UP4_TAATCGCGCAAGAGCGCCGG
• BKK28510 ([gene|1DF08A474E95909EAF68CF26D97DD49350ABEA89|abf2]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK28510 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGCCTCTGATCATTCCTT,  downstream forward: _UP4_TAATCGCGCAAGAGCGCCGG

References

• 14973026,18757805,20525796,22900538,18563919