ycnD

168

NADPH-FMN oxidoreductase, delivers reduced FMN to enzymes that require the reduced cofactor for activity

locus: BSU_03860

Molecular weight: 27.72 kDa

pI: 5.08

Protein length: 249 aa

Gene length: 750 bp

function: delivery of FMN to enzymes

product: NADPH-FMN oxidoreductase

essential: no

synonyms: ycnD

List of homologs in different organisms, belongs to COG0778 (Galperin et al., 2021)

Gene

Coordinates: 438,516 → 439,265

Advertisement

The protein

Catalyzed reaction/ biological activity

• FMNH₂ + NADP⁺ --> FMN + 2 H⁺ + NADPH (according to UniProt)
• FMNH₂ + NAD⁺ --> FMN + 2 H⁺ + NADH (according to UniProt)

Protein family

• flavin oxidoreductase frp family (with [protein|5B95F7CFEFCB954FCBC11087F030A1D777F34B7A|nfrA], according to UniProt)

[wiki|Cofactors]

• FMN or FAD [Pubmed|21635694]

Structure

• [PDB|1ZCH]
• [AF|P94424]

Expression and Regulation

Operons

• genes: [gene|C8B3C93479DC1EF6DAD6FCBEEBAD57817BBAFDDA|ycnE]-[gene|BA6C61C488659D5F1B5237BA817A8A5AC6D0E988|ycnD]

  description: [Pubmed|17407181]

  regulation

  • induced by chromanon [Pubmed|17407181]

Biological materials

Mutant

• MGNA-C013 (ycnD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2011 NBRP B. subtilis, Japan]
• BKE03860 (Δ[gene|BA6C61C488659D5F1B5237BA817A8A5AC6D0E988|ycnD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03860 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATGAGACACCCTTCCTT,  downstream forward: _UP4_TAAGCTCTTATTCGGCCTGT
• BKK03860 (Δ[gene|BA6C61C488659D5F1B5237BA817A8A5AC6D0E988|ycnD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03860 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATGAGACACCCTTCCTT,  downstream forward: _UP4_TAAGCTCTTATTCGGCCTGT

References

• 16229462,17407181