ydeA

168

glyoxalase III-like enzyme

locus: BSU_05110

Molecular weight: 22.31 kDa

pI: 5.64

Protein length: 197 aa

Gene length: 594 bp

function: detoxification of methylglyoxal

product: glyoxalase III-like enzyme

essential: no

ec: null

synonyms: ydeA

List of homologs in different organisms, belongs to COG0693 (Galperin et al., 2021)

Gene

Coordinates: 558,408  559,001

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The protein

Catalyzed reaction/ biological activity

• methylglyoxal - D-lactate [Pubmed|24330391]

Protein family

• [wiki|peptidase C56 family] (according to UniProt)

[wiki|Domains]

• [wiki|PfpI endopeptidase domain] (aa 29-166) (according to UniProt)

Structure

• [PDB|3F5D]
• [AF|P96658]

Biological materials

Mutant

• MGNA-C128 (ydeA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2126 NBRP B. subtilis, Japan]
• BKE05110 ([gene|36F6585A2EB990FE06A09AC56F2DB4E67CAEEE28|ydeA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE05110 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACAACTATATACCGCCTTA,  downstream forward: _UP4_TAATTTGCTGGAAATGATTT
• BKK05110 ([gene|36F6585A2EB990FE06A09AC56F2DB4E67CAEEE28|ydeA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK05110 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACAACTATATACCGCCTTA,  downstream forward: _UP4_TAATTTGCTGGAAATGATTT

References

• 24330391