ydeL

168

similar to transcriptional regulator ([wiki|MocR/ GabR family])

locus: BSU_05240

Molecular weight: 53.36 kDa

pI: 9.22

Protein length: 463 aa

Gene length: 1392 bp

function: unknown

product: unknown

essential: no

synonyms: ydeL

List of homologs in different organisms, belongs to COG1167 (Galperin et al., 2021)

Gene

Coordinates: 571,389  572,780

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The protein

Protein family

• [wiki|MocR/ GabR family ][Pubmed|22020104]
• [wiki|class-I pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)

[wiki|Domains]

• N-terminal DNA-binding helix-turn-helix motif; C-terminal domain is homologous to PLP-binding large domain of aminotransferases.
• [wiki|HTH gntR-type domain] (aa 13-81) (according to UniProt)

[wiki|Cofactors]

• PLP

Structure

• [PDB|4MGR] ([protein|C7C36FAC0CE72226960FC7E8E016B8EC77AF1036|gabR], 38% identity) [pubmed|24127574]
• [AF|P96669]

[wiki|Localization]

• nucleoid (Homogeneous) [Pubmed|16479537]

Biological materials

Mutant

• MGNA-C083 (ydeL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2081 NBRP B. subtilis, Japan]
• BKE05240 ([gene|05A23F4611674B0092262DEC309F7AD6B9B5BE30|ydeL]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE05240 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTGCGCGTTGTTCTCCT,  downstream forward: _UP4_TGAGGGAGCTTTCAGTTTAG
• BKK05240 ([gene|05A23F4611674B0092262DEC309F7AD6B9B5BE30|ydeL]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK05240 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTGCGCGTTGTTCTCCT,  downstream forward: _UP4_TGAGGGAGCTTTCAGTTTAG

References

• 11756427,16479537,21630458,22020104,24127574