ydfJ

168

phospholipid efflux pump

locus: BSU_05430

Molecular weight: 76.85 kDa

pI: 5.18

Protein length: 724 aa

Gene length: 2175 bp

function: resistance to the membrane-acting antibiotic rhodomyrtone

product: phospholipid efflux pump

essential: no

ec: null

synonyms: ydfJ, farE

List of homologs in different organisms, belongs to COG2409 (Galperin et al., 2021)

Gene

Coordinates: 589,717  591,891

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The protein

Catalyzed reaction/ biological activity

• export of phosphatidylglycerol-containing phospholipids

Protein family

• resistance-nodulation-cell division (RND) (TC 2.A.6) family (with [protein|1632474628BEA113F297897D152276A1AB73BAE8|ydgH] and [protein|AAC32230E3E585932A876737131C3E2C15168B2A|swrC], according to UniProt)

Structure

• [PDB|6N3T] (from Mycobacterium smegmatis, 25% identity) [pubmed|31113875]
• [AF|P96687]

[wiki|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|1B61298ECA583D2112AA56A88358E8A9A55D2C9D|ydfJ]

  description: [pubmed|22383849]

  regulatory mechanism

  • [protein|EFEAF09E4449A022A7323450FDED9458426A0080|ydfI]: activation, [Pubmed|15941986], in [regulon|protein:EFEAF09E4449A022A7323450FDED9458426A0080|ydfI regulon]

Biological materials

Mutant

• MGNA-C149 (ydfJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2147 NBRP B. subtilis, Japan]
• BKE05430 ([gene|1B61298ECA583D2112AA56A88358E8A9A55D2C9D|ydfJ]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE05430 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGATAATCTCCTTTCAAC,  downstream forward: _UP4_TAATAGAAAAAGCAGATCTT
• BKK05430 ([gene|1B61298ECA583D2112AA56A88358E8A9A55D2C9D|ydfJ]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK05430 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGATAATCTCCTTTCAAC,  downstream forward: _UP4_TAATAGAAAAAGCAGATCTT

References

• 15941986,31113875