yfhL

168

general stress protein, important for survival at low temperature (4C) and during ethanol stress

locus: BSU_08580

Molecular weight: 11.86 kDa

pI: 11.02

Protein length: 110 aa

Gene length: 333 bp

product: resistence protein (against toxic peptide [protein|820635420B3980B620F3E3D7ACF7EDE9DC6275AD|sdpC])

essential: no

synonyms: yfhL

List of homologs in different organisms, belongs to COG5658 (Galperin et al., 2021)

Gene

Coordinates: 929,406  929,738

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The protein

Structure

• [AF|O31580]

Paralogous protein(s)

• [protein|2E0FBCA66FB88EDFD345FA289F776C7CA23976F5|ybgB], [protein|3FB5839A7A80CF52E627FF1744E50490F5CC390F|sdpI]

[wiki|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|893103B8E6163A4B403CA136A92BA3EA66DC3802|yfhK]-[gene|2B7F65B145005A8ADD2A278E3EA5A52DF162C837|yfhL]-[gene|ED478E2C37F2AD8BF06251B52511287EDB91C9EE|yfhM]

  description: [Pubmed|10913081]

  regulation

  • induced by stress ([protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]) [Pubmed|10913081,15805528]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|10913081,15805528], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
  • [protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW]: sigma factor, [Pubmed|9987136], in [regulon|protein:3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW regulon]

Biological materials

Mutant

• MGNA-C364 (yfhL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2362 NBRP B. subtilis, Japan]
• BKE08580 ([gene|2B7F65B145005A8ADD2A278E3EA5A52DF162C837|yfhL]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE08580 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATCATCATCTCCTTTCT,  downstream forward: _UP4_ACGCACAGTCAAGGAGGAGA
• BKK08580 ([gene|2B7F65B145005A8ADD2A278E3EA5A52DF162C837|yfhL]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK08580 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATCATCATCTCCTTTCT,  downstream forward: _UP4_ACGCACAGTCAAGGAGGAGA

References

• 16629676,10913081,15805528,9987136