yfmL

yfmL
168

DEAD-box RNA helicase

locus
BSU_07430
Molecular weight
42.03 kDa
pI
9.6
Protein length
Gene length
function
RNA helicase
product
DEAD-box RNA helicase
essential
no
synonyms
yfmL

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0513 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
816,113  817,243
Phenotypes of a mutant
poor growth at low temperatures (16 to 20C) [Pubmed|23175651]
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The protein
Catalyzed reaction/ biological activity
RNA helicase
required for [wiki|ribosome] assembly [Pubmed|23175651]
ATP + H2O --> ADP + H+ + phosphate (according to UniProt)
Protein family
[wiki|helicase family] (according to UniProt)
[wiki|DEAD-box RNA helicases] (according to UniProt)
[wiki|Domains]
[wiki|Helicase ATP-binding domain] (aa 35-205) (according to UniProt)
[wiki|Helicase C-terminal domain] (aa 231-374) (according to UniProt)
Structure
[PDB|5IVL] ([protein|E169F9711635457EA65FA71CCE9D7FF29DD8DF02|cshA], 87% coverage, 35% identity) [Pubmed|28238534]
[AF|O34750]
Paralogous protein(s)
[protein|C83BD80DE0FD187D07B914F64696438ED5130107|cshB], [protein|E169F9711635457EA65FA71CCE9D7FF29DD8DF02|cshA], [protein|EACD0C84AA3CE41EF05EAA3D25AA1153293EB562|deaD]
Expression and Regulation
Operons
genes
[gene|0362D937D985846AEE4D0DEA57B62F915E8D35E5|yfmL]
description
[pubmed|22383849]
regulation
expression declines in the stationary phase [Pubmed|23175651]
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[gene|0362D937D985846AEE4D0DEA57B62F915E8D35E5|yfmL]

2024-03-11 10:31:12

Jstuelk

24

196ae6d1f911e8cb9511675864d798bf201444ca

B4271C5451E1D7D81F44805A6ACF01D03DD803BA

Biological materials
Mutant
MGNA-C239 ([gene|0362D937D985846AEE4D0DEA57B62F915E8D35E5|yfmL]::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2237 NBRP B. subtilis, Japan]
GP1053 ([gene|0362D937D985846AEE4D0DEA57B62F915E8D35E5|yfmL]::erm), available in the [wiki|Jörg Stülke]'s lab [pubmed|23175651]
BKE07430 ([gene|0362D937D985846AEE4D0DEA57B62F915E8D35E5|yfmL]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE07430 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTTGTTCTCCTCAATTC,  downstream forward: _UP4_TAAAAAGAAGGGCGAAGAGC
BKK07430 ([gene|0362D937D985846AEE4D0DEA57B62F915E8D35E5|yfmL]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK07430 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTTGTTCTCCTCAATTC,  downstream forward: _UP4_TAAAAAGAAGGGCGAAGAGC
Expression vectors
for expression/ purification from ''B. subtilis'' with C-terminal Strep-tag, for [wiki|SPINE], expression from the native chromomsomal site: GP1069 (spc), available in [wiki|Jörg Stülke]'s lab
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab, [pubmed|20572937]
FLAG-tag construct
GP1066 (spc, based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab
GFP fusion
B. subtilis GP1082 yfmL-yfp spc, available in [wiki|Jörg Stülke]'s lab
References
Reviews
38430409
Original Publications
23175651,28238534,30337909

0362D937D985846AEE4D0DEA57B62F915E8D35E5

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Time of last update: 2024-03-28 16:50:37

Author of last update: Jstuelk