yfnE

168

similar to chondroitin synthase

locus: BSU_07300

Molecular weight: 45.33 kDa

pI: 5.96

Protein length: 392 aa

Gene length: 1179 bp

function: unknown

product: unknown

essential: no

synonyms: yfnE

List of homologs in different organisms, belongs to COG0463 (Galperin et al., 2021)

Gene

Coordinates: 801,172 → 802,350

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The protein

Protein family

• [wiki|glycosyltransferase 2 family] (according to UniProt)

Structure

• [PDB|2Z86] (E. coli chondroitin synthase, corresponds to aa 4 ... 259, 25% identity) [pubmed|18771653]
• [AF|O06483]

Expression and Regulation

Operons

• genes: [gene|B25AE6979AF12628780C4489FFED966C6B31968A|yfnH]-[gene|9836E90BD443F5269A43E5C69FCF2CCB22EDF65B|yfnG]-[gene|4148A3F8E256D9B1D667C28428D867B5B6B5F3CE|yfnF]-[gene|E3481379D3FEC02C35CB4BE17A5246A208C5C86D|yfnE]-[gene|1D0286ECDA32FB21737170B54A57D3FE482C6422|yfnD]

  description: [Pubmed|22383849]

  regulation

  • expressed during sporulation ([protein|search|SigK]) [Pubmed|15383836]

  regulatory mechanism

  • [protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE]: activation, [Pubmed|26577401], in [regulon|protein:19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE regulon]

  sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK]: sigma factor, [Pubmed|15383836], in [regulon|protein:24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK regulon]
  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, internal promoter in [protein|E3481379D3FEC02C35CB4BE17A5246A208C5C86D|yfnE] open reading frame [Pubmed|15383836], see [http://dbtbs.hgc.jp/COG/prom/yfnHGFED.html DBTBS] for details, in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]

• genes: [gene|E3481379D3FEC02C35CB4BE17A5246A208C5C86D|yfnE]-[gene|1D0286ECDA32FB21737170B54A57D3FE482C6422|yfnD]

  description: [Pubmed|22383849]

  regulation

  • expressed during sporulation ([protein|search|SigK]) [Pubmed|15383836]

Biological materials

Mutant

• MGNA-C229 (yfnE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2227 NBRP B. subtilis, Japan]
• BKE07300 (Δ[gene|E3481379D3FEC02C35CB4BE17A5246A208C5C86D|yfnE]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE07300 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACTTCATTTTCACTTCCTG,  downstream forward: _UP4_CTTAGATCATTGGATGTGGT
• BKK07300 (Δ[gene|E3481379D3FEC02C35CB4BE17A5246A208C5C86D|yfnE]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK07300 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACTTCATTTTCACTTCCTG,  downstream forward: _UP4_CTTAGATCATTGGATGTGGT

References

• 15383836,18771653