yhcA

168

c-di-AMP exporter

locus: BSU_09010

Molecular weight: 58.17 kDa

pI: 9.49

Protein length: 532 aa

Gene length: 1599 bp

function: secretion of cyclic di-AMP

product: c-di-AMP exporter

essential: no

synonyms: yhcA

List of homologs in different organisms, belongs to COG2814 (Galperin et al., 2021)

Gene

Coordinates: 977,775  979,373

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The protein

Catalyzed reaction/ biological activity

• secretion of cyclic di-AMP [pubmed|29588402]

Protein family

• [wiki|major facilitator superfamily] (according to UniProt)
• [wiki|EmrB family] (according to UniProt)

Structure

• [PDB|6E8J] (from Hyphomonas neptunium, corresponds to aa 93 ... 204, 28% identity)
• [AF|P54585]

Paralogous protein(s)

• [protein|188742B9A0C95344D32A4C23B9C971660572CF7D|ycnB], [protein|23483430AE0381406EF2349A18CFAE1F14F9B180|lmrB]

[wiki|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|EDDD61CF725449E57C72F63D378F6CD1FAC49ADA|yhbI]-[gene|211C58A92BA2A06A34B1F73BDB68A0F581B942C2|yhbJ]-[gene|93159DC7D5C42CFA54F66F232D73F38957243AB7|yhcA]-[gene|7797B0B66922D51B9D142DD2E5857836E47F015B|yhcB]-[gene|95EED3A91483502CA78067421F678B1EDB965B18|yhcC]

  description: [Pubmed|22383849]

  additional information

  • the mRNA is substantially stabilized upon depletion of [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] (the half-life of the mRNA increases from 4 to 41 min) [PubMed|21815947]

Biological materials

Mutant

• MGNA-B471 (yhcA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1470 NBRP B. subtilis, Japan]
• GP1704 (''[gene|93159DC7D5C42CFA54F66F232D73F38957243AB7|yhcA]''::''ermC'', without terminator), available in [wiki|Jörg Stülke]'s lab
• BKE09010 ([gene|93159DC7D5C42CFA54F66F232D73F38957243AB7|yhcA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE09010 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTAAGACAACCTCCTTT,  downstream forward: _UP4_TAATCTGTACAGGGAGGTTT
• BKK09010 ([gene|93159DC7D5C42CFA54F66F232D73F38957243AB7|yhcA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK09010 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTAAGACAACCTCCTTT,  downstream forward: _UP4_TAATCTGTACAGGGAGGTTT

References

• 21815947,29588402