yitA

168

sulfate adenylyltransferase

locus: BSU_10920

Molecular weight: 43.09 kDa

pI: 7.55

Protein length: 389 aa

Gene length: 1170 bp

function: sulfate reduction and activation

product: sulfate adenylyltransferase

essential: no

ec: 2.7.7.4

synonyms: yitA

List of homologs in different organisms, belongs to COG2046 (Galperin et al., 2021)

Gene

Coordinates: 1,170,473 → 1,171,642

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The protein

Catalyzed reaction/ biological activity

• ATP + H⁺ + sulfate --> adenosine 5'-phosphosulfate + diphosphate (according to UniProt)

Protein family

• sulfate adenylyltransferase family (with [protein|6CF2A9A517B641C44C0B46EF242BC5E668EA846B|sat], according to UniProt)

Structure

• [PDB|1R6X] (from yeast, 41% identity) [pubmed|14983089]
• [AF|O06736]

Paralogous protein(s)

• [protein|6CF2A9A517B641C44C0B46EF242BC5E668EA846B|sat]

Expression and Regulation

Operons

• genes: [gene|E5090587E23B284EF3B3D8F3EA5C44CB86EEB82E|yitB]-[gene|EBBA4432FF83B6F203C694E04111D45290BE157A|yitA]-[gene|FE2B31E5EBE6C4BA4FC4AAC485A00C97315E6AAB|yisZ]

  description: [Pubmed|22383849]

  regulation

  • expressed during sporulation in the mother cell ([protein|search|SigK]) [Pubmed|15699190,15383836]

  sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK]: sigma factor, [Pubmed|15699190,15383836], in [regulon|protein:24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK regulon]

Biological materials

Mutant

• MGNA-A500 (yitA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/500 NBRP B. subtilis, Japan]
• BKE10920 (Δ[gene|EBBA4432FF83B6F203C694E04111D45290BE157A|yitA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10920 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAACGTGTTCAACCTCCATT,  downstream forward: _UP4_AAGCAAAACAGCGGGGAATT
• BKK10920 (Δ[gene|EBBA4432FF83B6F203C694E04111D45290BE157A|yitA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10920 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAACGTGTTCAACCTCCATT,  downstream forward: _UP4_AAGCAAAACAGCGGGGAATT

References

• 15699190,15383836,14983089