yjbA

yjbA
168

sporulation-specific adaptor protein, targets proteins to degradation by [protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC]-[protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP] during sporulation

locus
BSU_11410
Molecular weight
29.97 kDa
pI
5.15
Protein length
Gene length
function
control of [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]-dependent early spore gene expression
product
adaptor protein
essential
no
ec
null
synonyms
yjbA

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG5917 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,217,326  1,218,078
Phenotypes of a mutant
mutant: increased [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]-dependent gene expression during early sporulation [https://www.biorxiv.org/content/10.1101/2024.03.02.583065v1.full.pdf bioRxiv]
mutant: reduced sporulation (20 ... 30% of wild type) [https://www.biorxiv.org/content/10.1101/2024.03.02.583065v1.full.pdf bioRxiv]
overexpression of [protein|7CFDFB42AF816FF5766086E537472008E6CB7E85|yjbA]: toxic during vegetative growth, this can be suppressed by inactivation of [gene|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC] [https://www.biorxiv.org/content/10.1101/2024.03.02.583065v1.full.pdf bioRxiv]
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The protein
Protein family
UPF0736 family (single member, according to UniProt)
[wiki|Domains]
N-terminal domain: aa 1 ... 135 [https://www.biorxiv.org/content/10.1101/2024.03.02.583065v1.full.pdf bioRxiv]
C-terminal domain: aa 143 ... 250 [https://www.biorxiv.org/content/10.1101/2024.03.02.583065v1.full.pdf bioRxiv]
Structure
[PDB|8B3S] (the [protein|7CFDFB42AF816FF5766086E537472008E6CB7E85|yjbA]-[protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC] complex) [https://www.biorxiv.org/content/10.1101/2024.03.02.583065v1.full.pdf bioRxiv]
[AF|O31597]
[wiki|Localization]
peripheral forespore membrane protein [https://www.biorxiv.org/content/10.1101/2024.03.02.583065v1.full.pdf bioRxiv]
Expression and Regulation
Operons
genes
[gene|7CFDFB42AF816FF5766086E537472008E6CB7E85|yjbA]
description
[pubmed|22383849]
regulation
expressed from 2 hrs after onset of sporulation ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]) [https://www.biorxiv.org/content/10.1101/2024.03.02.583065v1.full.pdf bioRxiv]
sigma factors
[protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]: sigma factor, [Pubmed|16497325], in [regulon|protein:CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF regulon]
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[gene|7CFDFB42AF816FF5766086E537472008E6CB7E85|yjbA]

2024-03-21 22:10:08

Jstuelk

32

6C5F504559D866662578AD2FDD41D4DC8521A4EF

AA878BAF02EDC0AADFDE52F4CC5C852C3DDD4649

Biological materials
Mutant
MGNA-B149 (yjbA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1148 NBRP B. subtilis, Japan]
BKE11410 ([gene|7CFDFB42AF816FF5766086E537472008E6CB7E85|yjbA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE11410 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTCTAACCCCTCACTTT,  downstream forward: _UP4_TGAAAAAAACCGCTCTTTGC
BKK11410 ([gene|7CFDFB42AF816FF5766086E537472008E6CB7E85|yjbA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK11410 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTCTAACCCCTCACTTT,  downstream forward: _UP4_TGAAAAAAACCGCTCTTTGC
References
16497325,35638784

7CFDFB42AF816FF5766086E537472008E6CB7E85

Page visits: 1596

Time of last update: 2024-03-28 16:06:58

Author of last update: Jstuelk