yloB

yloB
168

ATP-driven Ca2+ pump, transports calcium ions from the mother cell to the forespore

locus
BSU_15650
Molecular weight
97.10 kDa
pI
8.71
Protein length
Gene length
function
accumulation of calcium in the spore
product
ATP-driven Ca2+ pump
essential
no
ec
3.6.3.8
synonyms
yloB

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0474 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,637,965  1,640,637
Phenotypes of a mutant
forms unstable spores [pubmed|30602489]
delayed spore [category|SW.4.2.4|Germination] [pubmed|30602489]
defective in [category|SW.4.1.2|Biofilm formation] [pubmed|35663026]
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The protein
Catalyzed reaction/ biological activity
transport of calcium ions from the mother cell to the forespore [pubmed|30602489]
there is no indication for an implication of [protein|7821A09DADC6EAF589A882F05CC34BE900E06F2A|yloB] in Mg2+ uptake (based on mutant phenotypes, failure to complement mutants defective in Mg2+ uptake) [Pubmed|24415722]
ATP + Ca2+ + H2O --> ADP + Ca2+ + H+ + phosphate (according to UniProt)
Protein family
[wiki|cation transport ATPase (P-type) (TC 3.A.3) family] (according to UniProt)
Structure
[PDB|4YCM] (the calcium pump with bound marine macrolide BLS from O. cuniculus, 35% identity) [pubmed|25957767]
[AF|O34431]
[wiki|Localization]
cell membrane [pubmed|30602489]
Expression and Regulation
Operons
genes
[gene|7821A09DADC6EAF589A882F05CC34BE900E06F2A|yloB]-[gene|A57D2C1536F3E93CD687C28C59FF297355CAB303|yloC]-[gene|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA]-[gene|1BC994DE60A7BB35DEDD7154581A396D29AA94A7|gmk]-[gene|921933B6B893083F151AABDEDCBC65F2E55677BE|yloH]
description
[pubmed|22383849]
regulation
expressed during sporulation in the mother cell [pubmed|12161109]
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[gene|7821A09DADC6EAF589A882F05CC34BE900E06F2A|yloB]->[gene|921933B6B893083F151AABDEDCBC65F2E55677BE|yloH]

2024-03-10 09:03:51

Jstuelk

94

b15c1f28629d3e15db14719be44c291dd5f28c5f

86CE620BD4E14CC259270F542702B302B7837CCD

Biological materials
Mutant
MGNA-B139 (yloB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1138 NBRP B. subtilis, Japan]
BKE15650 ([gene|7821A09DADC6EAF589A882F05CC34BE900E06F2A|yloB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15650 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCCGCTCGTCCACTCCC,  downstream forward: _UP4_TAAATTCATATGATATAATC
BKK15650 ([gene|7821A09DADC6EAF589A882F05CC34BE900E06F2A|yloB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15650 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCCGCTCGTCCACTCCC,  downstream forward: _UP4_TAAATTCATATGATATAATC
References
24415722,28951477,25957767,30602489,35663026

7821A09DADC6EAF589A882F05CC34BE900E06F2A

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Time of last update: 2024-03-28 05:22:01

Author of last update: Jstuelk