yloN

168

tRNA adenosine methyltransferase

locus: BSU_15750

Molecular weight: 41.41 kDa

pI: 6.79

Protein length: 363 aa

Gene length: 1092 bp

function: RNA modification

product: tRNA methyltransferase

essential: no

synonyms: yloN, rlmN

List of homologs in different organisms, belongs to COG0820 (Galperin et al., 2021)

Gene

Coordinates: 1,649,286  1,650,377

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The protein

Catalyzed reaction/ biological activity

• methylates C-2 in A2503 in 23S rRNA and A37 in tRNA [pubmed|23752511]
• adenosine²⁵⁰³ in 23S rRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine --> 2-methyladenosine²⁵⁰³ in 23S rRNA + 5'-deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-adenosyl-L-homocysteine (according to UniProt)
• adenosine³⁷ in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine --> 2-methyladenosine³⁷ in tRNA + 5'-deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-adenosyl-L-homocysteine (according to UniProt)

Protein family

• [wiki|Radical SAM superfamily] (according to UniProt)

[wiki|Cofactors]

• Fe-S cluster [pubmed|29292548]

Structure

• [PDB|3RF9] (from E. coli, 38% identity) [pubmed|21527678]
• [AF|O34617]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA]-[gene|06971E9AAB033E2878914A76645F99E97CE8A90A|fmt]-[gene|1370D8783450D538C71E3DE8EA5B37DFEE073FF0|yloM]-[gene|5DEBD84E5937DB8B7F29C729FAA0076DF86A5451|yloN]

  description: [Pubmed|16964327]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|16964327], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

• genes: [gene|A57D2C1536F3E93CD687C28C59FF297355CAB303|yloC]-[gene|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA]-[gene|1BC994DE60A7BB35DEDD7154581A396D29AA94A7|gmk]-[gene|921933B6B893083F151AABDEDCBC65F2E55677BE|yloH]-[gene|2B67C63442F28F6BCBA3BA6DE4B563C73E8DF16C|coaBC]-[gene|AF5ADDA2A98EC18BB72A81AA0336E639C0D1F430|priA]-[gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA]-[gene|06971E9AAB033E2878914A76645F99E97CE8A90A|fmt]-[gene|1370D8783450D538C71E3DE8EA5B37DFEE073FF0|yloM]-[gene|5DEBD84E5937DB8B7F29C729FAA0076DF86A5451|yloN]

  description

  • [pubmed|22383849]

Biological materials

Mutant

• MGNA-B132 (yloN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1131 NBRP B. subtilis, Japan]
• 1A819 ( ''yloN''::''erm''), [Pubmed|12682299], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A819&Search=1A819 BGSC]
• BKE15750 ([gene|5DEBD84E5937DB8B7F29C729FAA0076DF86A5451|yloN]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15750 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TGTTTTATTAAGTTCTGCCA,  downstream forward: _UP4_CAAGACGAGACGAGGTGATG
• BKK15750 ([gene|5DEBD84E5937DB8B7F29C729FAA0076DF86A5451|yloN]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15750 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TGTTTTATTAAGTTCTGCCA,  downstream forward: _UP4_CAAGACGAGACGAGGTGATG

References

• 18025251,18307109,27496281,23752511,11918677,21527678