yneA

168

inhibits cell division during SOS response

locus: BSU_17860

Molecular weight: 11.75 kDa

pI: 4.61

Protein length: 105 aa

Gene length: 318 bp

function: inhibits cell division during SOS response

product: checkpoint enforcement protein

essential: no

synonyms: yneA

List of homologs in different organisms

Gene

Coordinates: 1,918,406  1,918,723

Phenotypes of a mutant

• increased sensitivity to DNA-damaging agents [Pubmed|23728628]
• inactivation allows growth of a [gene|search|whiA ][gene|search|parB ]double mutant [pubmed|29378890]

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The protein

Protein family

• YneA family (single member, according to UniProt)

[wiki|Domains]

• contains a N-acetylglucosamine-polymer-binding [wiki|LysM domain] (aa 38-89) [Pubmed|20400548,18430080]

Structure

• [AF|Q45056]

[wiki|Localization]

• secreted to the medium (signal peptide) [Pubmed|20400548]

Additional information

• the activity can be bypassed by specific variants of [protein|8AB7D225DBEE6BD695A4A8A2384D2C029B571C57|ftsW] [pubmed|34097787]

Expression and Regulation

Operons

• genes: [gene|7BF591DCDC9635C605D76135481A8A9DB63EE861|yneA]-[gene|67074435B8FDA85C8ED4D8C4ECE3580C4EDFBF2A|yneB]-[gene|5BFD552F8A467C8D95FABE87CC8C5E7F04114886|ynzC]

  description: [Pubmed|12581363]

  regulation

  • induced by DNA damage ([protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]) [Pubmed|12581363]

  regulatory mechanism

  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]: repression, [Pubmed|12581363], in [regulon|protein:D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA regulon]

  additional information

  • YneA is rapidly degraded by extracellular proteases ([protein|5ED8301B9681FDD5171A9344A971E891493A6558|ctpA]) [PubMed|29979679,20400548]

Biological materials

Mutant

• MGNA-B388 (yneA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1387 NBRP B. subtilis, Japan]
• BKE17860 ([gene|7BF591DCDC9635C605D76135481A8A9DB63EE861|yneA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17860 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAACCTCCAACAGGAATG,  downstream forward: _UP4_AGATAGAGAGGAAAGAGAGA
• BKK17860 ([gene|7BF591DCDC9635C605D76135481A8A9DB63EE861|yneA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17860 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAACCTCCAACAGGAATG,  downstream forward: _UP4_AGATAGAGAGGAAAGAGAGA

References

Reviews

• 22933559,18430080

Original Publications

• 12581363,20400548,16267290,23728628,29378890,16549676,29979679,30315724,34097787