yobL

168

LXG toxin, non-specific metal-dependent RNase

locus: BSU_19000

Molecular weight: 67.59 kDa

pI: 6.5

Protein length: 600 aa

Gene length: 1803 bp

function: competetion with other bacteria in biofilms

product: LXG toxin, RNase

essential: no

ec: null

synonyms: yobL

List of homologs in different organisms, belongs to COG5444 (Galperin et al., 2021)

Gene

Coordinates: 2,071,754 → 2,073,556

Phenotypes of a mutant

• the mutant is outcompeted by wild type cells in biofilms [pubmed|34280190]

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The protein

[wiki|Domains]

• [wiki|LXG domain] (aa 1-235) (according to UniProt)

Structure

• [AF|O34330]

Biological materials

Mutant

• MGNA-A309 (yobL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/309 NBRP B. subtilis, Japan]
• BKE19000 (Δ[gene|D24F5B473B9CFAD960D877A8BA593939012177E2|yobL]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE19000 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATCATTCTCCTTCCTAG,  downstream forward: _UP4_TGAGGTGTTTATATGATTTA
• BKK19000 (Δ[gene|D24F5B473B9CFAD960D877A8BA593939012177E2|yobL]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK19000 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATCATTCTCCTTCCTAG,  downstream forward: _UP4_TGAGGTGTTTATATGATTTA

References

• 22200572,34280190