mrfB

168

Mg²⁺-dependent 3'-5' DNA exonuclease, part of a novel nucleotide excision repair pathway (with [protein|4D286C045A43FBF0F6E93E9E46AD7F25E35ADA7C|mrfA])

locus: BSU_22210

Molecular weight: 47.83 kDa

pI: 7.16

Protein length: 413 aa

Gene length: 1242 bp

function: mitomycin C-specific DNA damage repair

product: metal-dependent exonuclease

essential: no

synonyms: mrfB, yprB

List of homologs in different organisms, belongs to COG3359 (Galperin et al., 2021)

Gene

Coordinates: 2,333,324  2,334,565

Phenotypes of a mutant

• sensitive to mitomycin C [pubmed|30379365]

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The protein

[wiki|Cofactors]

• Mg²⁺ [pubmed|30379365]

Structure

• [PDB|8UN9] [pubmed|38405983]
• [AF|P50837]

Expression and Regulation

Operons

• genes: [gene|4D286C045A43FBF0F6E93E9E46AD7F25E35ADA7C|mrfA]-[gene|3F1D78CAAA4B5057DEBB24C4D382F96DA4AA4852|mrfB]-[gene|FA8D9AA0968365522DEFC05B277123FC96482044|cotD]

  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A413 (yprB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/413 NBRP B. subtilis, Japan]
• BKE22210 ([gene|3F1D78CAAA4B5057DEBB24C4D382F96DA4AA4852|mrfB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22210 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TAATGACATATCCGGCCTCC,  downstream forward: _UP4_TAAATATTCCCCGGGAAAGC
• BKK22210 ([gene|3F1D78CAAA4B5057DEBB24C4D382F96DA4AA4852|mrfB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22210 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TAATGACATATCCGGCCTCC,  downstream forward: _UP4_TAAATATTCCCCGGGAAAGC

References

Reviews

• 35210609

Research papers

• 30379365,38405983