yqeL

168

ribosomal silencing factor, dissociates the vacant 70S ribosomes during nutrient limitation

locus: BSU_25620

Molecular weight: 13.16 kDa

pI: 4.37

Protein length: 118 aa

Gene length: 357 bp

function: translation

product: ribosomal silencing factor

essential: no

synonyms: yqeL, rsfS

List of homologs in different organisms, belongs to COG0799 (Galperin et al., 2021)

Gene

Coordinates: 2,642,841 → 2,643,197

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The protein

Catalyzed reaction/ biological activity

• silences translation by binding to the L14 protein of the large ribosomal subunit and, as a consequence, impairs subunit joining  [Pubmed|22829778]
• dissociates the vacant 70S ribosomes during nutrient limitation [https://www.biorxiv.org/content/10.1101/2024.03.23.586405v1.full.pdf BioRxiv]

Protein family

• Iojap/RsfS family (single member, according to UniProt)

Structure

• [PDB|2O5A] (from B. halodurans, 65% identity)
• [AF|P54457]

[wiki|Interactions]

• [protein|2BE91EEB70A1961D3BBE0603648E64E86CD0B708|rplN]-[protein|B9D5E9B566D28423232C1A28C966CDEA0E5A6B78|yqeL] [Pubmed|22829778], the interaction serves to silence [wiki|translation] [Pubmed|22829778]

Additional information

• the protein is enriched in 45S assembly intermediates that accumulate upon depletion of [protein|54CD247926F7FABE4ACE85EB7021B62500B47260|rbgA] [Pubmed|23700310]

Expression and Regulation

Operons

• genes: [gene|722FD63050EED803C859FFD61338C02D5BB4CC1C|yqeG]-[gene|959C0ED7B9DA50F0EADCF1565405B878D36DA206|yqeH]-[gene|776D79C45DADA631D4A75B0773CFF471A87D9EEC|aroD]-[gene|7774FC36BAEAD33EDD48CB77DA0B5E7E0EA38CA2|yqeI]-[gene|AA4BB1F5B58371D536A6BBA86F6ECB5E5BFB12A8|nadD]-[gene|247C47AC70A0EA53E625871D9BC463A06E6B05FB|yqeK]-[gene|B9D5E9B566D28423232C1A28C966CDEA0E5A6B78|yqeL]-[gene|7F63251ABC07F6C11C57B62CBB948240B08D0181|yqeM]

  description: [Pubmed|22383849]

  expression

  • expression is increased upon depletion of depletion of tRNA maturation factors [gene|57A79AAF00243B5E058FA901D43AA7B55CA33F63|rnpB] or [gene|C40C5D35ED53D343C8200248FCCB010BAB388054|rnz] [pubmed|36840557]

Biological materials

Mutant

• MGNA-C500 (yqeL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2498 NBRP B. subtilis, Japan]
• BKE25620 (Δ[gene|B9D5E9B566D28423232C1A28C966CDEA0E5A6B78|yqeL]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE25620 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GTTCATTCACAAATTCCTCC,  downstream forward: _UP4_GATCTTGACTTTGGAATGAA
• BKK25620 (Δ[gene|B9D5E9B566D28423232C1A28C966CDEA0E5A6B78|yqeL]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK25620 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GTTCATTCACAAATTCCTCC,  downstream forward: _UP4_GATCTTGACTTTGGAATGAA

References

• 36815589 22829778,23700310,22383849