yqhH

168

similar to SNF2 helicase

locus: BSU_24580

Molecular weight: 64.50 kDa

pI: 8.38

Protein length: 557 aa

Gene length: 1674 bp

function: unknown

product: unknown

essential: no

synonyms: yqhH

List of homologs in different organisms, belongs to COG0553 (Galperin et al., 2021)

Gene

Coordinates: 2,549,775  2,551,448

Phenotypes of a mutant

• poorly transformable [pubmed|28189581]

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The protein

Protein family

• [wiki|helicase family] (according to UniProt)
• SNF2/RAD54 helicase family (with [protein|265490FA83D6D3F178C26C9240E3CB224513BF42|ywqA], according to UniProt)

[wiki|Domains]

• [wiki|Helicase ATP-binding domain] (aa 70-224) (according to UniProt)
• [wiki|Helicase C-terminal domain] (aa 363-524) (according to UniProt)

Structure

• [PDB|1Z6A] (from Sulfolobus solfataricus, 27% identity) [PDB|15882619]
• [AF|P54509]

Expression and Regulation

Operons

• genes: [gene|565D91F3E0357C5B16CC342FCDA7EFD4B910829C|yqhH]-[gene|22BEB39F735DAAF71F1B382CD453A9C2D4CC9FA4|yqhG]

  description

  • expressed early during sporulation

  regulation

  • expressed early during sporulation in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]) [Pubmed|16497325]

  sigma factors

  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]: sigma factor, [Pubmed|16497325], in [regulon|protein:CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF regulon]

Biological materials

Mutant

• MGNA-C418 (yqhH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2416 NBRP B. subtilis, Japan]
• BKE24580 ([gene|565D91F3E0357C5B16CC342FCDA7EFD4B910829C|yqhH]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24580 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTGCTTTGAAACCGCCTT,  downstream forward: _UP4_TAGCCTGTAAGGAGGTTTTA
• BKK24580 ([gene|565D91F3E0357C5B16CC342FCDA7EFD4B910829C|yqhH]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24580 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTGCTTTGAAACCGCCTT,  downstream forward: _UP4_TAGCCTGTAAGGAGGTTTTA

References

• 16497325,28189581,15882619