yqiD

168

geranyltransferase

locus: BSU_24280

Molecular weight: 29.59 kDa

pI: 5.02

Protein length: 296 aa

Gene length: 891 bp

function: biosynthesis of peptidoglycan and teichoic acid

product: geranyltransferase

essential: no

ec: 2.5.1.10

synonyms: yqiD, ispA

List of homologs in different organisms, belongs to COG0142 (Galperin et al., 2021)

Gene

Coordinates: 2,525,789 → 2,526,679

Phenotypes of a mutant

• The mutation of ''yqiD'' predisposes cells to grow without a wall [Pubmed|19212404]

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The protein

Catalyzed reaction/ biological activity

• (2E)-geranyl diphosphate + isopentenyl diphosphate --> (2E,6E)-farnesyl diphosphate + pp_i (according to UniProt)

Protein family

• FPP/GGPP synthase family (with [protein|3CAEA54E80A0B56EE120B881C8CDBE055AF6BDC1|hepT], according to UniProt)

[wiki|Cofactors]

• Magnesium

Structure

• [PDB|1RQI]: IspA from ''E. coli''.  Co-crystal with substrate isopentyl pyrophosphate and substrate analogue dimethyallyl S-thiolodiphosphate [Pubmed|14672944]
• [AF|P54383]

Modification

• None

Paralogous protein(s)

• [protein|3CAEA54E80A0B56EE120B881C8CDBE055AF6BDC1|hepT]

Biological materials

Mutant

• BKE24280 (Δ[gene|C75A4465688BCE9902F9CDC6DE069E36667377E1|yqiD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24280 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CCGGTCCGCCAGAAAGCTCG,  downstream forward: _UP4_TAAAACGATCCTAATTTGAA
• BKK24280 (Δ[gene|C75A4465688BCE9902F9CDC6DE069E36667377E1|yqiD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24280 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CCGGTCCGCCAGAAAGCTCG,  downstream forward: _UP4_TAAAACGATCCTAATTTGAA

References

• 14672944,17114254,19212404,17458547,23452849,26051891