brfA

168

release factor-dependent ribosome rescue factor

locus: BSU_23540

Molecular weight: 7.95 kDa

pI: 11.38

Protein length: 71 aa

Gene length: 216 bp

function: resolution of stalled translation complexes

product: ribosome rescue factor

essential: no

ec: null

synonyms: brfA, yqkK

List of homologs in different organisms

Gene

Coordinates: 2,451,148 → 2,451,363

Phenotypes of a mutant

• essential in the absence of trans-translation ([gene|5C12BB19B975F7FEBCFA119DAC66338C20FCECFA|ssrA], [gene|D19519B5A776D6E8EC2BFE7A7D67469CA0A6459A|smpB]) [pubmed|31776341]

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The protein

Catalyzed reaction/ biological activity

• binds to non-stop stalled ribosomes, recruits homologous [protein|12E06FD3A36D95AE82E3C65788FA98178039592F|RF2], but not [protein|59E6468FD891F5271F38543515867B0B4625768D|RF1], and induces its transition into an open active conformation [pubmed|31776341]

Structure

• [PDB|6SZS] [pubmed|31776341]
• [AF|P54573]

Biological materials

Mutant

• MGNA-C457 (yqkK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2455 NBRP B. subtilis, Japan]
• BKE23540 (Δ[gene|F7F780D11A6B11EB62CFA5202D1243A381AB40E1|brfA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23540 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTGATCACTCCCTTTTT,  downstream forward: _UP4_TAGCATTCATCTCTATTGTT
• BKK23540 (Δ[gene|F7F780D11A6B11EB62CFA5202D1243A381AB40E1|brfA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23540 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTGATCACTCCCTTTTT,  downstream forward: _UP4_TAGCATTCATCTCTATTGTT

References

Reviews

• 33815344

Research papers

• 31776341,37496314