forC2

168

formate:menadione oxidoreductase (with [protein|364BC3B51D8DF152C2DF31046D4F80595C75884B|forE2]), catalytic subunit

locus: BSU_27220

Molecular weight: 108.57 kDa

pI: 5.16

Protein length: 980 aa

Gene length: 2943 bp

function: unknown

product: formate:menadione oxidoreductase

essential: no

ec: 1.2.1.2

synonyms: yrhE

List of homologs in different organisms, belongs to COG3383 (Galperin et al., 2021)

Gene

Coordinates: 2,781,209  2,784,151

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The protein

Catalyzed reaction/ biological activity

• formate + NAD⁺ --> CO₂ + NADH (according to UniProt)

Protein family

• C-terminal part: [wiki|prokaryotic molybdopterin-containing oxidoreductase family] (according to UniProt)

[wiki|Domains]

• 2Fe-2S ferredoxin-type domain (aa 5-81) (according to UniProt)
• 4Fe-4S His(Cys)3-ligated-type domain (aa 81-121) (according to UniProt)
• 2 [wiki|4Fe-4S ferredoxin-type domain]s (aa 144-171, aa 187-216) (according to UniProt)
• [wiki|4Fe-4S Mo/W bis-MGD-type domain] (aa 263-319) (according to UniProt)

[wiki|Cofactors]

• 4 4Fe-4S cluster [pubmed|34748728]
• 1 2FE-2S cluster [pubmed|34748728]
• molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) [pubmed|34748728]
• menachinone-7 [pubmed|34748728]

Structure

• [PDB|2FUG] (from Thermus thermophilus, 23% identity) [pubmed|16469879]
• [AF|Q795Y4]

Modification

• Fe-S cluster [pubmed|29292548]

Paralogous protein(s)

• [protein|E54F39E72DC4881E886159607C2B424A41DE0D01|forC1]

Expression and Regulation

Operons

• genes: [gene|A4304213CFC152B601B2EED2B9B3A0A3AB4E6A81|forC2]-[gene|364BC3B51D8DF152C2DF31046D4F80595C75884B|forE2]

  description: [Pubmed|21815947]

  additional information

  • term-seq has identified a potential novel regulatory RNA element including an intrinsic transcription terminator upstream of [gene|search|yrhE] [Pubmed|27120414]

Biological materials

Mutant

• MGNA-A160 (yrhE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/160 NBRP B. subtilis, Japan]
• BKE27220 ([gene|A4304213CFC152B601B2EED2B9B3A0A3AB4E6A81|forC2]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27220 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTCATATTCCCCTCCA,  downstream forward: _UP4_GATTAAACGATAGGAGGAAA
• BKK27220 ([gene|A4304213CFC152B601B2EED2B9B3A0A3AB4E6A81|forC2]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27220 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTCATATTCCCCTCCA,  downstream forward: _UP4_GATTAAACGATAGGAGGAAA

References

• 21815947,27120414,16469879,34748728