ytoI

168

similar to transcriptional regulator ([wiki|GntR family])

locus: BSU_29270

Molecular weight: 48.87 kDa

pI: 6.59

Protein length: 439 aa

Gene length: 1320 bp

function: unknown

product: unknown

essential: no

synonyms: ytoI

List of homologs in different organisms, belongs to COG4109 (Galperin et al., 2021)

Gene

Coordinates: 2,997,301 → 2,998,620

Phenotypes of a mutant

• an ''ytoI'' point mutation has been isolated upon selction at low pressure [Pubmed|26296725]

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The protein

Protein family

• [wiki|GntR family] of transcription factors

[wiki|Domains]

• 2 [wiki|CBS domain]s (aa 195-254, aa 256-314) (according to UniProt)

Structure

• [AF|O34921]

Expression and Regulation

Operons

• genes: [gene|B4909B417F11C393A693488E07CC96B405FB1CE0|ytoI]

  description: [Pubmed|9387221]

  additional information

  • An [wiki|ncRNA] is predicted for'[protein|search|ytoI]' [PubMed|20525796]

Biological materials

Mutant

• MGNA-A152 (ytoI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/152 NBRP B. subtilis, Japan]
• GP2213 (''[gene|B4909B417F11C393A693488E07CC96B405FB1CE0|ytoI]''::''aphA3''), available in [wiki|Jörg Stülke]'s lab
• GP2214 (''[gene|B4909B417F11C393A693488E07CC96B405FB1CE0|ytoI]''::''phleo''), available in [wiki|Jörg Stülke]'s lab
• BKE29270 (Δ[gene|B4909B417F11C393A693488E07CC96B405FB1CE0|ytoI]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29270 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAGCCTTTCACCTCTAAGG,  downstream forward: _UP4_AGCTAAGGGACGTTCAGGCG
• BKK29270 (Δ[gene|B4909B417F11C393A693488E07CC96B405FB1CE0|ytoI]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29270 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAGCCTTTCACCTCTAAGG,  downstream forward: _UP4_AGCTAAGGGACGTTCAGGCG

Expression vectors

• pGP2933 (N-terminal His-tag, purification from ''E. coli'', in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab

References

• 16479537,9387221,20525796,23504016,26296725,31685546