ywdJ

168

unknown

locus: BSU_37940

Molecular weight: 49.81 kDa

pI: 10.02

Protein length: 440 aa

Gene length: 1323 bp

function: unknown

product: unknown

essential: no

ec: null

synonyms: ywdJ, ipa-60d

List of homologs in different organisms, belongs to COG2233 (Galperin et al., 2021)

Gene

Coordinates: 3,894,463 → 3,895,785

Advertisement

The protein

Protein family

• [wiki|xanthine/uracil permease family] (according to UniProt)
• [wiki|Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily] (according to UniProt)

Structure

• [AF|P39618]

[wiki|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|F267C75289D19D039F6E97F5DABCDFF0B4E4B8FA|ywdI]-[gene|BA4D8EF5B09345E94EEEEB936C803B71340DC5EE|ywdJ]-[gene|386D4F640E987E3752BEBE57BF25DF487D12EFCE|ywdK]

  description: [Pubmed|12823818]

  regulation

  • repressed in the absence of good nitrogen sources (glutamine or ammonium) ([protein|search|TnrA]) [Pubmed|12823818]

  regulatory mechanism

  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]: repression, [Pubmed|12823818], in [regulon|protein:857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA regulon]

Biological materials

Mutant

• MGNA-A565 (ywdJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/565 NBRP B. subtilis, Japan]
• BKE37940 (Δ[gene|BA4D8EF5B09345E94EEEEB936C803B71340DC5EE|ywdJ]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE37940 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTTCACGTAAGCTCAACCTC,  downstream forward: _UP4_TGAATTTTGGTGCAGGTGCG
• BKK37940 (Δ[gene|BA4D8EF5B09345E94EEEEB936C803B71340DC5EE|ywdJ]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK37940 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTTCACGTAAGCTCAACCTC,  downstream forward: _UP4_TGAATTTTGGTGCAGGTGCG
• BP1259 ([gene|BA4D8EF5B09345E94EEEEB936C803B71340DC5EE|ywdJ]::cat trp+) available in [wiki|Jörg Stülke]'s & [wiki|Fabian Commichau]'s lab

References

• 12823818,25755103