ywhA

168

similar to transcriptional regulator ([wiki|MarR family])

locus: BSU_37550

Molecular weight: 15.73 kDa

pI: 8.14

Protein length: 139 aa

Gene length: 420 bp

function: unknown

product: unknown

essential: no

synonyms: ywhA

List of homologs in different organisms, belongs to COG1846 (Galperin et al., 2021)

Gene

Coordinates: 3,853,717  3,854,136

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The protein

[wiki|Domains]

• [wiki|HTH marR-type domain] (aa 8-139) (according to UniProt)

Structure

• [AF|P70993]

Expression and Regulation

Operons

• genes: [gene|CA74CB721842927F67FBC3B5C27D2EE46B30CE1C|thrZ]-[gene|4A3CA47533B217F595E7B600923DA31228B94DA8|ywhA]

  description: [Pubmed|1379177]

  regulation

  • induced by threonine limitation ([wiki|T-box]) [Pubmed|19258532]
  • the [wiki|T-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]

  regulatory mechanism

  • T-box: RNA switch, in [regulon|other_regulator:T-box|T-box]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|1379177], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• MGNA-A517 (ywhA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/517 NBRP B. subtilis, Japan]
• BKE37550 ([gene|4A3CA47533B217F595E7B600923DA31228B94DA8|ywhA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE37550 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTCAATTTCCCCATTTC,  downstream forward: _UP4_TAGGGATAATAAAAGCCGGT
• BKK37550 ([gene|4A3CA47533B217F595E7B600923DA31228B94DA8|ywhA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK37550 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTCAATTTCCCCATTTC,  downstream forward: _UP4_TAGGGATAATAAAAGCCGGT

References

• 1379177,19258532