ywjA

168

similar to [wiki|ABC transporter] (ATP-binding protein)

locus: BSU_37230

Molecular weight: 64.39 kDa

pI: 6.14

Protein length: 575 aa

Gene length: 1728 bp

function: unknown

product: [wiki|ABC transporter] (ATP-binding protein)

essential: no

synonyms: ywjA

List of homologs in different organisms, belongs to COG1132 (Galperin et al., 2021)

Gene

Coordinates: 3,819,754 → 3,821,481

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The protein

Protein family

• [wiki|ABC transporter superfamily] (according to UniProt)

[wiki|Domains]

• has both a membrane-spanning and an ATP-binding domain [Pubmed|10092453]
• [wiki|ABC transmembrane type-1 domain] (aa 16-299) (according to UniProt)
• [wiki|ABC transporter domain] (aa 333-567) (according to UniProt)

Structure

• [PDB|2HYD] (from Staphylococcus aureus, 38% identity) [pubmed|16943773]
• [AF|P45861]

Paralogous protein(s)

• [protein|E85814EDF232D21A42380D559EAD2CDFB41F19DB|yfiC], [protein|ED1F82463BAA28B67184BFAB5CB23CF26A62999D|bmrA], [protein|70D7098BEBD5E53B4B9B8C3394FAAF972C65EE22|yknU], [protein|C6B0ACB9D9703DD8141FD7D9D62DB22FB48F458B|yknV], [protein|0EB4D3E1B9AF39ECCBD79EB559FAE5BB0EE4C156|ygaD], [protein|67C0C9EA17EF953554D3D57CD0D285246F50C1AD|bmrC], [protein|288CA73D93B1E5106E1C6CE5E7E2E8BE2A6BD3F3|yfiB]

[wiki|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|07752EC6940F43DEBD1E9313A2A195E35EDB9F69|ywiE]-[gene|D5A3C316BD4567A0155F70330E32ACDE18F063FE|ywjA]-[gene|685B58AD2A02A0B48B7D4AAAE046DE2CF8F77C58|ywjB]

  description: [pubmed|22383849]

  regulatory mechanism

  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]: repression, [pubmed|12354229], in [regulon|protein:F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur regulon]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|26577401], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]

• genes: [gene|D5A3C316BD4567A0155F70330E32ACDE18F063FE|ywjA]-[gene|685B58AD2A02A0B48B7D4AAAE046DE2CF8F77C58|ywjB]

  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-B662 (ywjA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1661 NBRP B. subtilis, Japan]
• BKE37230 (Δ[gene|D5A3C316BD4567A0155F70330E32ACDE18F063FE|ywjA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE37230 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACCCTATCTTCCCCCAATG,  downstream forward: _UP4_ATGGTGCATCGATAGGAGGA
• BKK37230 (Δ[gene|D5A3C316BD4567A0155F70330E32ACDE18F063FE|ywjA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK37230 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACCCTATCTTCCCCCAATG,  downstream forward: _UP4_ATGGTGCATCGATAGGAGGA

References

• 10092453,12354229,26577401,16943773