yxeB

168

hydroxamate siderophore [wiki|ABC transporter] (only ferrioxamine) (binding protein)

locus: BSU_39610

Molecular weight: 35.10 kDa

pI: 5.53

Protein length: 321 aa

Gene length: 966 bp

function: siderophore uptake

product: hydroxamate siderophore [wiki|ABC transporter]

essential: no

synonyms: yxeB

List of homologs in different organisms, belongs to COG0614 (Galperin et al., 2021)

Gene

Coordinates: 4,067,183  4,068,148

Phenotypes of a mutant

• no growth with the xenosiderophore ferrioxamine E as single source of iron [Pubmed|23220087]

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The protein

Protein family

• [wiki|Bacterial solute-binding protein 8 family] (according to UniProt)

[wiki|Domains]

• [wiki|Fe/B12 periplasmic-binding domain] (aa 58-316) (according to UniProt)

Structure

• [PDB|4FNA] ([protein|84CC89907EF39770F5BFACA82A11B04CF76EC5AA|fhuD] from Staphylococcus aureus, 35% identity) [pubmed|24606332]
• [AF|P54941]

Paralogous protein(s)

• [protein|84CC89907EF39770F5BFACA82A11B04CF76EC5AA|fhuD]

[wiki|Localization]

• extracellular (signal peptide) [Pubmed|18957862]
• membrane, lipid modification as retention signal [Pubmed|18763711]

Expression and Regulation

Operons

• genes: [gene|6F4F7A68701FAC3983AF447F11BC1EAB17D7B07B|yxeB]

  description: [pubmed|12354229]

  regulation

  • repressed unless the cells enter an iron starvation ([protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]) [Pubmed|12354229]

  regulatory mechanism

  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]: repression, [pubmed|12354229], in [regulon|protein:F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur regulon]

Biological materials

Mutant

• MGNA-B706 (yxeB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1705 NBRP B. subtilis, Japan]
• BKE39610 ([gene|6F4F7A68701FAC3983AF447F11BC1EAB17D7B07B|yxeB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE39610 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCAAGAACCCTCCCACTA,  downstream forward: _UP4_TAAAAAAACGGCACAGTCAT
• BKK39610 ([gene|6F4F7A68701FAC3983AF447F11BC1EAB17D7B07B|yxeB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK39610 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCAAGAACCCTCCCACTA,  downstream forward: _UP4_TAAAAAAACGGCACAGTCAT

References

• 10092453,16672620,12354229,18957862,18763711,23220087,24606332