Difference between revisions of "LevE"

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Revision as of 16:11, 2 July 2009

  • Description: trigger enzyme: fructose-specific phosphotransferase system, EIIB component

Gene name levE
Synonyms
Essential no
Product trigger enzyme: fructose-specific phosphotransferase system, EIIB component

fructose-specific enzyme IIB (EC 2.7.1.69)

Function fructose uptake and phosphorylation, control of LevR activity
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism
MW, pI 17 kDa, 9.444
Gene length, protein length 486 bp, 162 aa
Immediate neighbours levF, levD
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
LevE context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU27060

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate (according to Swiss-Prot)
  • Protein family: PTS permease, mannose permease (Man) family PubMed
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • KEGG entry: [2]

Additional information

Expression and regulation

  • Regulation: repressed by glucose (CcpA) , carbon catabolite repression, induction by fructose
  • Regulatory mechanism: CcpA: transcription repression, catabolite repression: transcription repression by CcpA, transcription activator LevR is less active in the presence of glucose; induction: transcription activation by LevR
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Jonathan Reizer, Steffi Bachem, Aiala Reizer, Maryvonne Arnaud, Milton H Saier, Jörg Stülke
Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 12);3419-3429
[PubMed:10627040] [WorldCat.org] [DOI] (P p)

S Schauder, R S Nunn, R Lanz, B Erni, T Schirmer
Crystal structure of the IIB subunit of a fructose permease (IIBLev) from Bacillus subtilis.
J Mol Biol: 1998, 276(3);591-602
[PubMed:9551099] [WorldCat.org] [DOI] (P p)

V Charrier, J Deutscher, A Galinier, I Martin-Verstraete
Protein phosphorylation chain of a Bacillus subtilis fructose-specific phosphotransferase system and its participation in regulation of the expression of the lev operon.
Biochemistry: 1997, 36(5);1163-72
[PubMed:9033408] [WorldCat.org] [DOI] (P p)

S Seip, R Lanz, R Gutknecht, K Flükiger, B Erni
The fructose transporter of Bacillus subtilis encoded by the lev operon: backbone assignment and secondary structure of the IIB(Lev) subunit.
Eur J Biochem: 1997, 243(1-2);306-14
[PubMed:9030753] [WorldCat.org] [DOI] (P p)

I Martin-Verstraete, M Débarbouillé, A Klier, G Rapoport
Levanase operon of Bacillus subtilis includes a fructose-specific phosphotransferase system regulating the expression of the operon.
J Mol Biol: 1990, 214(3);657-71
[PubMed:2117666] [WorldCat.org] [DOI] (P p)