Difference between revisions of "Buk"

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=Expression and regulation=
 
=Expression and regulation=
 
+
* '''Operon:''' ''[[ptb]]-[[bcd]]-[[buk]]-[[lpdV]]-[[bkdAA]]-[[bkdAB]]-[[bkdB]]'' {{PubMed|12823818}}
* '''Operon:''' ''[[ptb]]-[[bcd]]-[[buk]]-[[lpdV]]-[[bkdAA]]-[[bkdAB]]-[[bkdB]]''
 
  
 
* '''[[Sigma factor]]:''' [[SigL]] [http://www.ncbi.nlm.nih.gov/sites/entrez/10094682 PubMed]
 
* '''[[Sigma factor]]:''' [[SigL]] [http://www.ncbi.nlm.nih.gov/sites/entrez/10094682 PubMed]
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
** repressed during growth in the presence of branched chain amino acids ([[CodY]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/10094682 PubMed]
+
** induced in the presence of isoleucine or valine ([[BkdR]]) {{PubMed|10094682}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
 
** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/10094682 PubMed]
 
** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/10094682 PubMed]
 +
** [[BkdR]]: transcription activation {{PubMed|10094682}}
  
 
* '''Additional information:'''
 
* '''Additional information:'''
 +
  
 
=Biological materials =
 
=Biological materials =
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=References=
 
=References=
  
<pubmed>12427936,15241682,10094682,10094682, </pubmed>
+
<pubmed>12427936,15241682,10094682,12823818, </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 21:28, 19 November 2009

  • Description: butyrate kinase

Gene name buk
Synonyms yqiU, bkd
Essential no
Product butyrate kinase
Function utilization of branched-chain keto acids
Metabolic function and regulation of this protein in SubtiPathways:
Ile, Leu, Val
MW, pI 39 kDa, 5.466
Gene length, protein length 1089 bp, 363 aa
Immediate neighbours lpdV, bcd
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Buk context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU24070

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + butanoate = ADP + butanoyl phosphate (according to Swiss-Prot)
  • Protein family: acetokinase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • induced in the presence of isoleucine or valine (BkdR) PubMed
  • Additional information:


Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

M Nickel, G Homuth, C Böhnisch, U Mäder, T Schweder
Cold induction of the Bacillus subtilis bkd operon is mediated by increased mRNA stability.
Mol Genet Genomics: 2004, 272(1);98-107
[PubMed:15241682] [WorldCat.org] [DOI] (P p)

Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818] [WorldCat.org] [DOI] (P p)

Tanja Kaan, Georg Homuth, Ulrike Mäder, Julia Bandow, Thomas Schweder
Genome-wide transcriptional profiling of the Bacillus subtilis cold-shock response.
Microbiology (Reading): 2002, 148(Pt 11);3441-3455
[PubMed:12427936] [WorldCat.org] [DOI] (P p)

M Debarbouille, R Gardan, M Arnaud, G Rapoport
Role of bkdR, a transcriptional activator of the sigL-dependent isoleucine and valine degradation pathway in Bacillus subtilis.
J Bacteriol: 1999, 181(7);2059-66
[PubMed:10094682] [WorldCat.org] [DOI] (P p)