Difference between revisions of "CysI"

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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 +
* unable to grow with sulfate or sulfite as the only sulfur source {{PubMed|12169591}}
  
 
=== Database entries ===
 
=== Database entries ===
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=Expression and regulation=
 
=Expression and regulation=
 +
* '''Operon:''' ''[[cysI]]-[[cysJ]]'' {{PubMed|12169591}}
  
* '''Operon:''' ''[[cysJ]]-[[cysI]]''
+
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|12169591}}
  
* '''[[Sigma factor]]:''' [[SigA]]
+
* '''Regulation:'''  
 
+
** induced by sulfite, sulfate, and thiosulfate ([[CysL]]) {{PubMed|12169591}}
* '''Regulation:''' repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed]   
+
** repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed]   
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
 +
** [[CysL]]: transcription activation {{PubMed|12169591}}
  
 
* '''Additional information:'''
 
* '''Additional information:'''

Revision as of 08:18, 29 December 2009

  • Description: sulfite reductase (NADPH2)

Gene name cysI
Synonyms yvgR
Essential no
Product sulfite reductase (NADPH2)
Function sulfite reduction
Metabolic function and regulation of this protein in SubtiPathways:
Cys, Met & Sulfate assimilation
MW, pI 67 kDa, 4.573
Gene length, protein length 1815 bp, 605 aa
Immediate neighbours cysJ, helD
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YvgR context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU33440

Phenotypes of a mutant

  • unable to grow with sulfate or sulfite as the only sulfur source PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • induced by sulfite, sulfate, and thiosulfate (CysL) PubMed
    • repressed by casamino acids PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Isabelle Guillouard, Sandrine Auger, Marie-Françoise Hullo, Farid Chetouani, Antoine Danchin, Isabelle Martin-Verstraete
Identification of Bacillus subtilis CysL, a regulator of the cysJI operon, which encodes sulfite reductase.
J Bacteriol: 2002, 184(17);4681-9
[PubMed:12169591] [WorldCat.org] [DOI] (P p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

J R van der Ploeg, M Barone, T Leisinger
Functional analysis of the Bacillus subtilis cysK and cysJI genes.
FEMS Microbiol Lett: 2001, 201(1);29-35
[PubMed:11445163] [WorldCat.org] [DOI] (P p)