Difference between revisions of "Ald"

From SubtiWiki
Jump to: navigation, search
(Database entries)
Line 78: Line 78:
 
=== Database entries ===
 
=== Database entries ===
  
* '''Structure:'''
+
* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=2EEZ 2EEZ] (from ''Thermus thermophilus hb8 mutant'', 55% identity, 66% similarity)
 +
 
  
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/Q08352 Q08352]
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/Q08352 Q08352]

Revision as of 15:34, 17 February 2010

  • Description: L-alanine dehydrogenase

Gene name ald
Synonyms spoVN
Essential no
Product L-alanine dehydrogenase
Function alanine utilization
Metabolic function and regulation of this protein in SubtiPathways:
Ala, Gly, Ser
MW, pI 39 kDa, 5.125
Gene length, protein length 1134 bp, 378 aa
Immediate neighbours yukF, yukJ
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Ald context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU31930

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: L-alanine + H2O + NAD+ = pyruvate + NH3 + NADH (according to Swiss-Prot)
  • Protein family: AlaDH/PNT family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure: 2EEZ (from Thermus thermophilus hb8 mutant, 55% identity, 66% similarity)


  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Jessica C Wilks, Ryan D Kitko, Sarah H Cleeton, Grace E Lee, Chinagozi S Ugwu, Brian D Jones, Sandra S BonDurant, Joan L Slonczewski
Acid and base stress and transcriptomic responses in Bacillus subtilis.
Appl Environ Microbiol: 2009, 75(4);981-90
[PubMed:19114526] [WorldCat.org] [DOI] (I p)

Le Thi Tam, Christine Eymann, Haike Antelmann, Dirk Albrecht, Michael Hecker
Global gene expression profiling of Bacillus subtilis in response to ammonium and tryptophan starvation as revealed by transcriptome and proteome analysis.
J Mol Microbiol Biotechnol: 2007, 12(1-2);121-30
[PubMed:17183219] [WorldCat.org] [DOI] (P p)

Bauke Oudega, Micheline Vandenbol, Gregory Koningstein
A 12 kb nucleotide sequence containing the alanine dehydrogenase gene at 279 degrees on the Bacillus subtilis chromosome.
Microbiology (Reading): 1997, 143 ( Pt 5);1489-1491
[PubMed:9168598] [WorldCat.org] [DOI] (P p)

K J Siranosian, K Ireton, A D Grossman
Alanine dehydrogenase (ald) is required for normal sporulation in Bacillus subtilis.
J Bacteriol: 1993, 175(21);6789-96
[PubMed:8226620] [WorldCat.org] [DOI] (P p)