Difference between revisions of "YvcJ"

From SubtiWiki
Jump to: navigation, search
(Biological materials)
Line 110: Line 110:
 
* '''GFP fusion:'''
 
* '''GFP fusion:'''
  
* '''two-hybrid system:'''  
+
* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Boris Görke| Görke]] lab
  
 
* '''Antibody:'''
 
* '''Antibody:'''

Revision as of 06:31, 10 March 2010

  • Description: nucleotide-binding protein

Gene name yvcJ
Synonyms
Essential no
Product nucleotide-binding P-loop-containing protein
Function required for the expression of late competence genes
MW, pI 33 kDa, 6.702
Gene length, protein length 885 bp, 295 aa
Immediate neighbours yvcK, yvcI
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YvcJ context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU34770

Phenotypes of a mutant

reduced competence PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: binds and hydrolyzes nucleotides, required for the expression of late competence genes (comK, comS) PubMed
  • Protein family: UPF0042 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: very weak stimuation of expression by citrate and succinate PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector: pGP735 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab
  • Antibody:

Labs working on this gene/protein

Anne Galinier, University of Marseille, France

Your additional remarks

References

Jennifer Luciano, Elodie Foulquier, Jean-Raphael Fantino, Anne Galinier, Frédérique Pompeo
Characterization of YvcJ, a conserved P-loop-containing protein, and its implication in competence in Bacillus subtilis.
J Bacteriol: 2009, 191(5);1556-64
[PubMed:19074378] [WorldCat.org] [DOI] (I p)

Boris Görke, Elodie Foulquier, Anne Galinier
YvcK of Bacillus subtilis is required for a normal cell shape and for growth on Krebs cycle intermediates and substrates of the pentose phosphate pathway.
Microbiology (Reading): 2005, 151(Pt 11);3777-3791
[PubMed:16272399] [WorldCat.org] [DOI] (P p)

A Galinier, J Haiech, M C Kilhoffer, M Jaquinod, J Stülke, J Deutscher, I Martin-Verstraete
The Bacillus subtilis crh gene encodes a HPr-like protein involved in carbon catabolite repression.
Proc Natl Acad Sci U S A: 1997, 94(16);8439-44
[PubMed:9237995] [WorldCat.org] [DOI] (P p)