Difference between revisions of "Pgi"

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(Expression and regulation)
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* '''Mutant:''' GP508 (spc), available in [[Stülke]] lab
 
* '''Mutant:''' GP508 (spc), available in [[Stülke]] lab
  
* '''Expression vector:''' pGP762 (N-terminal His-tag, in [[pWH844]]), available in [[Stülke]] lab
+
* '''Expression vector:'''  
 +
 
 +
* '''pGP398 (N-terminal His-tag, in [[pWH844]]), available in [[Stülke]] lab
 
 
 
* '''lacZ fusion:''' pGP510 (in [[pAC6]]), available in [[Stülke]] lab
 
* '''lacZ fusion:''' pGP510 (in [[pAC6]]), available in [[Stülke]] lab

Revision as of 22:00, 18 March 2010

  • Description: glucose 6-phosphate isomerase, glycolytic / gluconeogenic enzyme

Gene name pgi
Synonyms yugL
Essential no
Product glucose-6-phosphate isomerase
Function enzyme in glycolysis / gluconeogenesis
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism, Sugar catabolism
MW, pI 50.4 kDa, 4.85
Gene length, protein length 1353 bp, 451 amino acids
Immediate neighbours yugM, yugK
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Pgi context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU31350

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: D-glucose 6-phosphate = D-fructose 6-phosphate (according to Swiss-Prot) D-glucose 6-phosphate = D-fructose 6-phosphate
  • Protein family: GPI family (according to Swiss-Prot) GPI family
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information: Reversible Michaelis-Menten PubMed
  • Domains:
  • Modification: phosphorylation on Thr-39 PubMed
  • Cofactor(s):
  • Effectors of protein activity: competitively inhibited by 6-phosphogluconate (in B.caldotenax, B.stearothermophilus) PubMed
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot), cytoplasm

Database entries

  • Structure: 1B0Z (Geobacillus stearothermophilus), 2PGI (Geobacillus stearothermophilus)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation: constitutively expressed PubMed
  • Additional information:

Biological materials

  • Mutant: GP508 (spc), available in Stülke lab
  • Expression vector:
  • pGP398 (N-terminal His-tag, in pWH844), available in Stülke lab
  • lacZ fusion: pGP510 (in pAC6), available in Stülke lab
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • Antibody:

Labs working on this gene/protein

Jörg Stülke, University of Göttingen, Germany homepage

Your additional remarks

References

PubMed